N -[4-(6-二甲氨基-2-苯并呋喃基)苯基]马来酰亚胺高效液相色谱法测定血清中d -青霉胺

Q4 Biochemistry, Genetics and Molecular Biology

Japanese Journal of Clinical Chemistry Pub Date : 1988-10-31 DOI:10.14921/JSCC1971B.17.2_51

K. Nakashima, Miyuki Muraoka, S. Nakatsuji, S. Akiyama

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引用次数: 3

摘要

建立了一种高效液相色谱法测定血清中d -青霉胺的方法。该方法是将d -青霉胺与N-[4-(6-二甲氨基-2-苯并呋喃基)苯基]马来酰亚胺进行荧光衍生化，并采用高效液相色谱法进行荧光检测。d -青霉胺的校准图在200 ~ 12 pmol范围内呈线性关系，在信噪比为2时的检测下限为50 fmol。2.9 ƒÊM的回收率为91.8% (C.V.=4.2%， n=5)。对3例妇女单次口服d -青霉胺胶囊后血清d -青霉胺水平的时间变化进行了观察。2小时后血药浓度达到最高值，24小时后逐渐下降。该方法可用于血液中d -青霉胺的监测。

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Determination of D-Penicillamine in Serum by High-Performance Liquid Chromatography Using N -[4-(6-Dimethylamino-2-benzofuranyl) phenyl] maleimide

A sensitive and simple high-performance liquid chromatographic determination method for D-penicillamine in serum was developed. The method is based on the fluorescence derivazation of D-penicillamine with N-[4-(6-dimethylamino-2-benzofuranyl)phenyl]maleimide and following fluorescence detection by high-performance liquid chromatography . A calibration graph for D-penicillamine was linear over the range from 200 fmol to 12 pmol , and the lower limit of detection at a signal-to-noise ratio of 2 was 50 fmol. Recovery rate of 2.9 ƒÊM of D-penicillamine was 91.8% (C.V.=4.2%, n=5). Time courses of serum levels of D-penicillamine after single oral administration of D-penicilamine in capsule to three women were examined. Serum levels reached maximum after 2 hr and then decreased gradually till 24 hr. This method will be useful for a monitoring of D-penicillamine in blood.

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Japanese Journal of Clinical Chemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

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