Bacteriophages of Bacillus subtilis

INTRODUCTION .............................................. 258 MAJOR GROUPS OF B. SUBTILIS PHAGES ....... .............. 258 Virulent Phages ............................................. 258 IMU-containing phages ......................................... 258 SP50, 41, 42, and 414 ........................................... 260 f22 ........... .................................. 260 429 and related phages ......................................... 261 SPP1 ............................................. 265 SP3 ........... .................................. 265 TSP-i ............. ................................ 265 Other virulent phages .......................................... 266 Temperate B. subtilis Phages ................ .................... 266 Transducing Phages and Pseudolysogeny ........ ................. 267 SPlo ............. ................................ 267 PBS1 and PBS2 ............................................. 268 SP15 ............. ................................ 269 Transduction with SPlO, PBS1, and SP15 ....... ................ 269 Defective Phages ............................................. 270 HOST-RELATED FUNCTIONS ................ .................... 271 Common Hosts ............................................. 271 Phage Receptor Sites ............................................ 273 Abortive Infections ............................................. 274 Physiological State of the Host and Entrapment ...... ............ 277 Mixed Bacteriophage Infections ............. ..................... 278 GENETICS OF B. SUBTILIS PHAGES ......... .................. 279 Types of Mutants ............................................. 279 Plaque morphology and host range mutants ....... .............. 279 Conditional lethal mutants .................. ................... 279 Genetic Maps ............................................. 280 HMU-containing phages ......................................... 280 029 ............................................. 281 4105 and SP02 ............................................. 282 Transfection ............................................. 283 Competence for transfection and transformation ...... .......... 284 Entry of transfecting DNA..................................... 284 Intracellular inactivation ....................................... 284 Role of host enzymes in transfection ......... ................... 287 Use of transfection to analyze phage development ..... ......... 289 SYNTHESIS OF MACROMOLECULES DURING THE DEVELOPMENT OF B. SUBTILIS PHAGES .......... ................ 289 Development of HMU-Containing Phages .......... ............... 290 DNA replication (i) Nucleotide metabolism ............... 290 (ii) DNA synthesis .......................................... 290 (iii) Arrest of host DNA synthesis ............ .................. 291 RNA synthesis (i) Program of transcription ......... ................ 292 (ii) Regulation of transcription ............. ................... 294 (iii) Synthesis of host RNA ...................................... 295 Protein synthesis (i) In vivo synthesis .............................. 296 (ii) In vitro synthesis .......................... 296 Development of 429 ........................... 297 DNA replication ............................................... 297