直接电刺激对受刺激和未受刺激对侧比目鱼肌神经肌肉连接形态的影响

IF 8.9 1区医学

Journal of Cachexia, Sarcopenia and Muscle Pub Date : 2023-04-15 DOI:10.1002/jcsm.13235

Young il Lee, Nicola Cacciani, Ya Wen, Xiang Zhang, Yvette Hedstr?m, Wesley Thompson, Lars Larsson

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引用次数: 0

摘要

越来越多的证据表明器官之间存在着串扰。神经肌肉连接(NMJ)是一种外周化学突触，其功能和形态对乙酰胆碱(ACh)释放和肌肉去极化敏感。为了提高我们对NMJ可塑性和肌肉串扰的理解，我们研究了长期突触后神经肌肉阻断大鼠后肢肌肉单侧直接电刺激对NMJ的影响。方法采用α-蛇毒全身给药并机械通气8 d，对Sprague Dawley大鼠进行突触后神经肌肉传递阻断，并与未治疗的假手术对照组和单侧慢性电刺激12 h/d，连续5、8 d进行比较。结果NMJs在突触后神经肌肉阻断反应中产生轴突和胶质芽(生长过程超出了由乙酰胆碱受体[achr]高密度聚集体定义的突触范围)，但在外周去神经支配或突触前阻断后较少报道。直接电刺激比目鱼肌可减少受刺激和未受刺激对侧比目鱼肌的末端雪旺细胞(tSC)和轴突萌发。8 d慢性刺激减少(P <0.001)，受刺激和未受刺激的比目鱼肌上的tSC芽数分别为6.7±0.5和6.9±0.5个，而受刺激的比目鱼肌上的tSC芽数为10.3±0.9个(P <0.001)，固定8天的大鼠无刺激比目鱼肌。轴突芽的类似减少(P <0.001)对侧受刺激和非受刺激比目鱼肌对慢性电刺激的反应。基于rnaseq的基因表达分析证实了对受刺激和未受刺激的对侧肌肉的恢复作用。这种交叉效应与受刺激和对侧未受刺激肌肉以及血浆中细胞因子/趋化因子水平的增加相平行。结论突触后神经肌肉阻断大鼠NMJs的运动轴突终末和末端雪旺细胞出现萌芽反应。这些轴突和神经胶质反应可能被一种肌肉来源的肌因子以活动依赖的方式释放，具有局部和全身效应。

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Direct electrical stimulation impacts on neuromuscular junction morphology on both stimulated and unstimulated contralateral soleus

There is increasing evidence of crosstalk between organs. The neuromuscular junction (NMJ) is a peripheral chemical synapse whose function and morphology are sensitive to acetylcholine (ACh) release and muscle depolarization. In an attempt to improve our understanding of NMJ plasticity and muscle crosstalk, the effects of unilateral direct electrical stimulation of a hindlimb muscle on the NMJ were investigated in rats exposed long‐term post‐synaptic neuromuscular blockade.

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来源期刊

Journal of Cachexia, Sarcopenia and Muscle Medicine-Orthopedics and Sports Medicine

自引率

12.40%

发文量

期刊介绍： The Journal of Cachexia, Sarcopenia, and Muscle is a prestigious, peer-reviewed international publication committed to disseminating research and clinical insights pertaining to cachexia, sarcopenia, body composition, and the physiological and pathophysiological alterations occurring throughout the lifespan and in various illnesses across the spectrum of life sciences. This journal serves as a valuable resource for physicians, biochemists, biologists, dieticians, pharmacologists, and students alike.