封面:蛋白质组学分析确定IRSp53和fastin是PRV输出和直接细胞-细胞传播的关键

IF 3.4 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Proteomics Pub Date : 2019-12-02 DOI:10.1002/pmic.201970201

Fei-Long Yu, Huan Miao, Jinjin Xia, Fan Jia, Huadong Wang, Fuqiang Xu, Lin Guo

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引用次数: 0

摘要

DOI: 10.1002 / pmic。在第1900009号文章中，Yu Fei-Long等人基于病毒粒子纯化策略对成熟的假狂犬病毒(PRV)细胞外和细胞内病毒粒子进行了全面的蛋白质组学分析。通过流式细胞术分选方法从感染的培养细胞中分离出PRV病毒粒子，并对细胞外和细胞内PRV蛋白质组进行表征和比较。封面上的双荧光图像显示假狂犬病毒(PRV)的包膜糖蛋白M-GFP和衣壳蛋白VP26-RFP上的标签。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Front Cover: Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

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Front Cover: Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

DOI: 10.1002/pmic.201900009

In article number 1900009, Fei-Long Yu et al. provide a comprehensive proteomics analysis of mature extracellular and intracellular Pseudorabies virus (PRV) virions based on a virion purification strategy. PRV virions are isolated from infected culture cells through a flow cytometry sorting-based method, and the extracellular and intracellular PRV proteomes are characterized and compared. The cover depicts a dual fluorescent image showing Pseudorabies viruses (PRV) with tags on the envelope glycoprotein M-GFP and the capsid protein VP26-RFP.

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来源期刊

Proteomics 生物-生化研究方法

CiteScore

6.30

自引率

5.90%

发文量

193

审稿时长

3 months

期刊介绍： PROTEOMICS is the premier international source for information on all aspects of applications and technologies, including software, in proteomics and other "omics". The journal includes but is not limited to proteomics, genomics, transcriptomics, metabolomics and lipidomics, and systems biology approaches. Papers describing novel applications of proteomics and integration of multi-omics data and approaches are especially welcome.