高效液相色谱法测定流动相中乙腈体积分数

IF 3.1 4区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Research in Chinese Universities Pub Date : 2008-11-01 DOI:10.1016/S1005-9040(09)60008-8

Yi WU , Zhi-wu WANG , Jing-kai GU , Ying-wu WANG

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引用次数: 4

摘要

本文报道了一种高效液相色谱法测定循环流动相中乙腈的方法的建立和验证。该方法是基于反相液相色谱的保留随着流动相中有机相浓度的降低而增加。给定溶质的容量比的自然对数与有机改性剂在流动相中的体积分数呈线性关系。对于邻苯二甲酸二甲酯和邻苯二甲酸二乙酯，线性范围为30%-60%，对于联苯和terphenyl，线性范围为60%-95%。精密度(RSD)均为±1%，准确度(RE)在±1%范围内。本实验成功地应用于柱状洗脱液蒸馏后循环流动相乙腈浓度的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Determination of Acetonitrile Volume Fraction in Mobile Phase by HPLC

This paper reports the development and validation of an assay for the determination of acetonitrile in the recycled mobile phase using high performance liquid chromatography(HPLC). The method is based on that the retention in reversed-phase liquid chromatography increases with decreasing concentration of organic phase in the mobile phase. The natural logarithm of the capacity ratio for a given solute is linearly related to the volume fraction of the organic modifier in the mobile phase. For dimethylphthalate and diethylphthalate, the linearity range is 30%–60%, and for biphenyl and terphenyl, the range is 60%–95%. Precision values(RSD) were both <1% and the accuracy(RE) was in the range of ±1%. The assay was successfully applied to the determination of acetonitrile concentration of recycled mobile phase after the distillation of the column eluent in our laboratory.

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来源期刊

Chemical Research in Chinese Universities 化学-化学综合

CiteScore

5.30

自引率

6.50%

发文量

152

审稿时长

3.0 months

期刊介绍： The journal publishes research articles, letters/communications and reviews written by faculty members, researchers and postgraduates in universities, colleges and research institutes all over China and overseas. It reports the latest and most creative results of important fundamental research in all aspects of chemistry and of developments with significant consequences across subdisciplines. Main research areas include (but are not limited to): Organic chemistry (synthesis, characterization, and application); Inorganic chemistry (bio-inorganic chemistry, inorganic material chemistry); Analytical chemistry (especially chemometrics and the application of instrumental analysis and spectroscopy); Physical chemistry (mechanisms, catalysis, thermodynamics and dynamics); Polymer chemistry and polymer physics (mechanisms, material, catalysis, thermodynamics and dynamics); Quantum chemistry (quantum mechanical theory, quantum partition function, quantum statistical mechanics); Biochemistry; Biochemical engineering; Medicinal chemistry; Nanoscience (nanochemistry, nanomaterials).