Seung-Il Jung MD, PhD , Hyun-Suk Lee MS , Hyung-Seok Kim MD, PhD , Kyuyoun Ahn MD, PhD , Kwangsung Park MD, PhD
{"title":"去势对腹侧前列腺水通道蛋白表达及细胞凋亡的影响","authors":"Seung-Il Jung MD, PhD , Hyun-Suk Lee MS , Hyung-Seok Kim MD, PhD , Kyuyoun Ahn MD, PhD , Kwangsung Park MD, PhD","doi":"10.1016/j.jomh.2012.03.015","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Aquaporins<span> (AQPs) are transmembrane proteins that play a major role in the regulation of membrane water permeability. The purpose of this study was to determine the expression and localization of AQPs in the ventral prostate (VP) and to predict a possible role for AQPs in apoptotic rat VP, as there has been little research on the expression of AQPs in rat prostate.</span></p></div><div><h3>Methods</h3><p><span>Male Sprague–Dawley rats were divided into two groups: a control group and a castration group. The expression and cellular localization of AQPs 1–9 in the VP were determined using immunochemistry<span> and Western blot analysis, respectively. </span></span>Apoptosis of the VP was determined using Western blot analysis of Bax and Bcl-2 proteins.</p></div><div><h3>Results</h3><p>Among the AQPs, AQP1 and 9 were expressed in rat VP. AQP9 expression was localized on the epithelial cell membranes in the intermediate region, which has a secretary function. The expression of AQP1 was not changed after castration; however, the expression of AQP9 was significantly decreased. Apoptosis of the epithelium developed on day 1 after castration and reached a maximum on day 3. At that time, the expression of AQP9 was highly preserved.</p></div><div><h3>Conclusions</h3><p>In rat VP, only AQP9 was expressed on the epithelial cell membrane in the intermediate region, which has a secretary function, implying that AQP9 may play a role in secretory processes. The apoptotic process and AQP9 expression was correlated, so further study of the role of AQPs in castration-induced apoptotic and transition processes will be required.</p></div>","PeriodicalId":54391,"journal":{"name":"Journal of Mens Health","volume":"9 4","pages":"Pages 238-244"},"PeriodicalIF":0.6000,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jomh.2012.03.015","citationCount":"2","resultStr":"{\"title\":\"Effects of castration on the expression of aquaporins and apoptosis in the ventral prostate\",\"authors\":\"Seung-Il Jung MD, PhD , Hyun-Suk Lee MS , Hyung-Seok Kim MD, PhD , Kyuyoun Ahn MD, PhD , Kwangsung Park MD, PhD\",\"doi\":\"10.1016/j.jomh.2012.03.015\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Aquaporins<span> (AQPs) are transmembrane proteins that play a major role in the regulation of membrane water permeability. The purpose of this study was to determine the expression and localization of AQPs in the ventral prostate (VP) and to predict a possible role for AQPs in apoptotic rat VP, as there has been little research on the expression of AQPs in rat prostate.</span></p></div><div><h3>Methods</h3><p><span>Male Sprague–Dawley rats were divided into two groups: a control group and a castration group. The expression and cellular localization of AQPs 1–9 in the VP were determined using immunochemistry<span> and Western blot analysis, respectively. </span></span>Apoptosis of the VP was determined using Western blot analysis of Bax and Bcl-2 proteins.</p></div><div><h3>Results</h3><p>Among the AQPs, AQP1 and 9 were expressed in rat VP. AQP9 expression was localized on the epithelial cell membranes in the intermediate region, which has a secretary function. The expression of AQP1 was not changed after castration; however, the expression of AQP9 was significantly decreased. Apoptosis of the epithelium developed on day 1 after castration and reached a maximum on day 3. At that time, the expression of AQP9 was highly preserved.</p></div><div><h3>Conclusions</h3><p>In rat VP, only AQP9 was expressed on the epithelial cell membrane in the intermediate region, which has a secretary function, implying that AQP9 may play a role in secretory processes. The apoptotic process and AQP9 expression was correlated, so further study of the role of AQPs in castration-induced apoptotic and transition processes will be required.</p></div>\",\"PeriodicalId\":54391,\"journal\":{\"name\":\"Journal of Mens Health\",\"volume\":\"9 4\",\"pages\":\"Pages 238-244\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2012-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jomh.2012.03.015\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Mens Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1875686712000619\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Mens Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1875686712000619","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Effects of castration on the expression of aquaporins and apoptosis in the ventral prostate
Background
Aquaporins (AQPs) are transmembrane proteins that play a major role in the regulation of membrane water permeability. The purpose of this study was to determine the expression and localization of AQPs in the ventral prostate (VP) and to predict a possible role for AQPs in apoptotic rat VP, as there has been little research on the expression of AQPs in rat prostate.
Methods
Male Sprague–Dawley rats were divided into two groups: a control group and a castration group. The expression and cellular localization of AQPs 1–9 in the VP were determined using immunochemistry and Western blot analysis, respectively. Apoptosis of the VP was determined using Western blot analysis of Bax and Bcl-2 proteins.
Results
Among the AQPs, AQP1 and 9 were expressed in rat VP. AQP9 expression was localized on the epithelial cell membranes in the intermediate region, which has a secretary function. The expression of AQP1 was not changed after castration; however, the expression of AQP9 was significantly decreased. Apoptosis of the epithelium developed on day 1 after castration and reached a maximum on day 3. At that time, the expression of AQP9 was highly preserved.
Conclusions
In rat VP, only AQP9 was expressed on the epithelial cell membrane in the intermediate region, which has a secretary function, implying that AQP9 may play a role in secretory processes. The apoptotic process and AQP9 expression was correlated, so further study of the role of AQPs in castration-induced apoptotic and transition processes will be required.
期刊介绍：
JOMH is an international, peer-reviewed, open access journal. JOMH publishes cutting-edge advances in a wide range of diseases and conditions, including diagnostic procedures, therapeutic management strategies, and innovative clinical research in gender-based biology. It also addresses sexual disparities in health, life expectancy, lifestyle and behaviors and so on. Scientists are encouraged to publish their experimental, theoretical, and descriptive studies and observations in as much detail as possible.
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