两性偶氮染料比较荧光凝胶电泳(CoFGE)的pi控制

Q4 Biochemistry, Genetics and Molecular Biology

EuPA Open Proteomics Pub Date : 2015-09-01 DOI:10.1016/j.euprot.2015.03.003

Marina Hanneken , Karel Šlais , Simone König

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引用次数: 4

摘要

凝胶与凝胶之间的差异是二维凝胶电泳的主要问题。垂直和水平比较荧光凝胶电泳(v/hCoFGE)最近被引入，以提高蛋白质点分配的再现性。在1D和2D-PAGE的组合中，蛋白质分析物被覆盖在参考网格上，允许纠正y方向的变化。然而，对第一个维度pI (x方向)的控制到目前为止还缺失。在这里，我们使用两性偶氮染料来完成CoFGE工具包。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes

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pI-Control in comparative fluorescence gel electrophoresis (CoFGE) using amphoteric azo dyes

Gel-to-gel variation is a major problem in two-dimensional (2D) gel electrophoresis. Vertical and horizontal comparative fluorescence gel electrophoresis (v/hCoFGE) were recently introduced to improve the reproducibility of protein spot assignment. There, in a combination of 1D- and 2D-PAGE, protein analytes were overlaid with a reference grid which allowed correcting for variation in the y-direction. However, control of the first dimension, pI (x-direction), was missing so far. Here, we applied amphoteric azo dyes for this purpose completing the CoFGE toolkit.

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来源期刊

EuPA Open Proteomics Biochemistry, Genetics and Molecular Biology-Biochemistry

自引率

0.00%

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审稿时长

103 days