在头颈部鳞状细胞癌中,淋巴结或神经周围浸润与miR-15a、miR-34c和miR-199b低水平相关

Lucas O. Sousa , Lays M. Sobral , Camila S. Matsumoto , Fabiano P. Saggioro , Rossana V.M. López , Rodrigo A. Panepucci , Carlos Curti , Wilson A. Silva Jr , Lewis J. Greene , Andréia M. Leopoldino
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Nine miRs (miR-15a, miR-21, miR-29b, miR-34c, miR-100, miR-125b, miR-137, miR-133b and miR-199b) were measured by real time qRT-PCR in HNSCC samples from 32 patients and eight resection margins. SET (I2PP2A) and PTEN protein levels were estimated by immunohistochemistry in paired HNSCC tissues and their matched resection margins.</p></div><div><h3>Results</h3><p>In HNSCC, the presence of lymph node invasion was associated with low miR-15a, miR-34c and miR-199b levels, whereas the presence of perineural invasion was associated with low miR-199b levels. In addition, miR-21 levels were high whereas miR-100 and miR-125b levels were low in HNSCC compared to the resection margins. 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引用次数: 22

摘要

micrornas (miRNAs或miRs)是真核细胞的转录后调节因子,对miR水平差异的了解可能为诊断和治疗提供新的方法。方法本研究测定头颈部鳞状细胞癌(HNSCC)中9种miR水平,并确定临床病理特征是否与miR水平差异相关。还测量了SET (I2PP2A)和PTEN蛋白水平,因为它们的水平分别可由miR-199b和miR-21调节。通过实时qRT-PCR检测来自32例患者和8个切除缘的HNSCC样本中的9种miRs (miR-15a, miR-21, miR-29b, miR-34c, miR-100, miR-125b, miR-137, miR-133b和miR-199b)。通过免疫组织化学方法估计配对HNSCC组织及其匹配切除边缘的SET (I2PP2A)和PTEN蛋白水平。结果在HNSCC中,淋巴结浸润与miR-15a、miR-34c和miR-199b低水平相关,而神经周围浸润与miR-199b低水平相关。此外,与切除边缘相比,HNSCC中miR-21水平较高,而miR-100和miR-125b水平较低。当转染miR-34c抑制剂或miR-34c模拟物转染HNSCC细胞系HN12时,无论是否敲低SET, miR-34c抑制剂均增加细胞侵袭能力,而miR-34c模拟物则降低细胞侵袭能力。结论肿瘤组织中特异性miRs的水平可以为HNSCC的维持和进展提供线索。在癌症的发生和发展过程中,mirna被上调或下调;可作为HNSCC的预后指标和治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lymph node or perineural invasion is associated with low miR-15a, miR-34c and miR-199b levels in head and neck squamous cell carcinoma

Lymph node or perineural invasion is associated with low miR-15a, miR-34c and miR-199b levels in head and neck squamous cell carcinoma

Background

MicroRNAs (miRNAs or miRs) are post-transcriptional regulators of eukaryotic cells and knowledge of differences in miR levels may provide new approaches to diagnosis and therapy.

Methods

The present study measured the levels of nine miRs in head and neck squamous cell carcinomas (HNSCC) and determined whether clinical pathological features are associated with differences in miR levels. SET (I2PP2A) and PTEN protein levels were also measured, since their levels can be regulated by miR-199b and miR-21, respectively. Nine miRs (miR-15a, miR-21, miR-29b, miR-34c, miR-100, miR-125b, miR-137, miR-133b and miR-199b) were measured by real time qRT-PCR in HNSCC samples from 32 patients and eight resection margins. SET (I2PP2A) and PTEN protein levels were estimated by immunohistochemistry in paired HNSCC tissues and their matched resection margins.

Results

In HNSCC, the presence of lymph node invasion was associated with low miR-15a, miR-34c and miR-199b levels, whereas the presence of perineural invasion was associated with low miR-199b levels. In addition, miR-21 levels were high whereas miR-100 and miR-125b levels were low in HNSCC compared to the resection margins. When HNSCC line HN12, with or without knockdown of SET, were transfected with miR-34c inhibitor or miR-34c mimic, the miR-34c inhibitor increased cell invasion capacity while miR-34c mimic decreased the cell invasion.

Conclusions

We showed that the levels of specific miRs in tumor tissue can provide insight into the maintenance and progression of HNSCC.

General significance

MiRNAs are up- or down-regulated during cancer development and progression; they can be prognosis markers and therapeutic targets in HNSCC.

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