数字PCR:简史

Q1 Biochemistry, Genetics and Molecular Biology

Biomolecular Detection and Quantification Pub Date : 2014-09-01 DOI:10.1016/j.bdq.2014.06.001

Alexander A. Morley

{"title":"数字PCR:简史","authors":"Alexander A. Morley","doi":"10.1016/j.bdq.2014.06.001","DOIUrl":null,"url":null,"abstract":"<div><p>Digital PCR for quantification of a target of interest has been independently developed several times, being described in 1990 and 1991 using the term “limiting dilution PCR” and in 1999 using the term “digital PCR”. It came into use in the decade following its first development but its use was cut short by the description of real-time PCR in 1996. However digital PCR has now had a renaissance due to the recent development of new instruments and chemistry which have made it a much simpler and more practical technique.</p></div>","PeriodicalId":38073,"journal":{"name":"Biomolecular Detection and Quantification","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bdq.2014.06.001","citationCount":"117","resultStr":"{\"title\":\"Digital PCR: A brief history\",\"authors\":\"Alexander A. Morley\",\"doi\":\"10.1016/j.bdq.2014.06.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Digital PCR for quantification of a target of interest has been independently developed several times, being described in 1990 and 1991 using the term “limiting dilution PCR” and in 1999 using the term “digital PCR”. It came into use in the decade following its first development but its use was cut short by the description of real-time PCR in 1996. However digital PCR has now had a renaissance due to the recent development of new instruments and chemistry which have made it a much simpler and more practical technique.</p></div>\",\"PeriodicalId\":38073,\"journal\":{\"name\":\"Biomolecular Detection and Quantification\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.bdq.2014.06.001\",\"citationCount\":\"117\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomolecular Detection and Quantification\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214753514000023\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomolecular Detection and Quantification","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214753514000023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 117

摘要

用于定量感兴趣目标的数字PCR已经独立开发了几次，在1990年和1991年使用术语“极限稀释PCR”进行描述，1999年使用术语“数字PCR”进行描述。它在首次开发后的十年中开始使用，但1996年实时PCR的描述缩短了它的使用。然而，由于最近新仪器和化学的发展，数字PCR现在已经复兴，这使它成为一种更简单、更实用的技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Digital PCR: A brief history

Digital PCR for quantification of a target of interest has been independently developed several times, being described in 1990 and 1991 using the term “limiting dilution PCR” and in 1999 using the term “digital PCR”. It came into use in the decade following its first development but its use was cut short by the description of real-time PCR in 1996. However digital PCR has now had a renaissance due to the recent development of new instruments and chemistry which have made it a much simpler and more practical technique.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biomolecular Detection and Quantification Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

14.20

自引率

0.00%

发文量

审稿时长

8 weeks