扫描电镜分析SARS-CoV-2与Vero细胞系的相互作用

IF 1.8 4区生物学 Q3 BIOPHYSICS

Journal of Biological Physics Pub Date : 2023-06-30 DOI:10.1007/s10867-023-09638-y

Zuzana Malá, Marek Vojta, Jan Loskot, Radek Sleha, Bruno Ježek, Josef Zelenka

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引用次数: 0

摘要

本研究采用扫描电子显微镜(SEM)研究了SARS-CoV-2感染细胞的细胞结构。我们的测量揭示了感染引起的感染重塑，包括在细胞膜上发生病毒形态发生的新的专门区域的出现。还观察到病毒细胞冲浪的细胞间扩展。我们的研究结果扩大了对SARS-CoV-2与细胞相互作用、细胞间传播以及细胞大小分布的认识。我们的研究结果表明，扫描电镜是一种有用的显微镜方法，可以分析细胞内的超微结构，显示出特定的表面修饰，也可以应用于研究其他重要的生物过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Analysis of SARS-CoV-2 interactions with the Vero cell lines by scanning electron microscopy

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Analysis of SARS-CoV-2 interactions with the Vero cell lines by scanning electron microscopy

In this study, scanning electron microscopy (SEM) was used to study the cell structure of SARS-CoV-2 infected cells. Our measurements revealed infection remodeling caused by infection, including the emergence of new specialized areas where viral morphogenesis occurs at the cell membrane. Intercellular extensions for viral cell surfing have also been observed. Our results expand knowledge of SARS-CoV-2 interactions with cells, its spread from cell to cell, and their size distribution. Our findings suggest that SEM is a useful microscopic method for intracellular ultrastructure analysis of cells exhibiting specific surface modifications that could also be applied to studying other important biological processes.

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来源期刊

Journal of Biological Physics 生物-生物物理

CiteScore

3.00

自引率

5.60%

发文量

审稿时长

>12 weeks

期刊介绍： Many physicists are turning their attention to domains that were not traditionally part of physics and are applying the sophisticated tools of theoretical, computational and experimental physics to investigate biological processes, systems and materials. The Journal of Biological Physics provides a medium where this growing community of scientists can publish its results and discuss its aims and methods. It welcomes papers which use the tools of physics in an innovative way to study biological problems, as well as research aimed at providing a better understanding of the physical principles underlying biological processes.