向GenBank提交序列

Current Protocols Essential Laboratory Techniques Pub Date : 2009-06-15 DOI:10.1002/9780470089941.et1102s01

Wei-Jen Chang, Thomas W. Coppola

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引用次数: 6

摘要

在后基因组时代，越来越多的研究项目涉及到分子序列数据的生成。如何分析这些新获得的DNA/蛋白质序列，如何准备提交到序列数据库?在本单元中，我们提供指南和流程图，以帮助首次用户使用第三方免费软件程序处理新的序列数据，并使用Sequin程序逐步演示准备提交给GenBank的序列文件。咕咕叫。Protoc。基本的实验室。科技,1:11.2.1-11.2.20。©2009 by John Wiley &儿子,Inc。

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Submitting a Sequence to GenBank

In the post-genomic era, more and more research projects involve the generation of molecular sequence data. How should these newly obtained DNA/protein sequences be analyzed, and how should they be prepared for submission to sequence databases? In this unit, we provide guidelines and a flow chart to help first-time users process new sequence data using third-party freeware programs and give a step-by-step demonstration on the preparation of a sequence file for submission to GenBank using the Sequin program. Curr. Protoc. Essential Lab. Tech. 1:11.2.1-11.2.20. © 2009 by John Wiley & Sons, Inc.

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Current Protocols Essential Laboratory Techniques

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