绿色合成纳米银及其抑菌活性研究。

IF 4.7 Q2 NANOSCIENCE & NANOTECHNOLOGY

Micro and Nano Systems Letters Pub Date : 2022-01-20 DOI:10.1186/s40486-022-00144-9

Ankush Sharma, Anand Sagar, Jagriti Rana, Reena Rani

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引用次数: 8

摘要

研究了利用红豆杉(Taxus baccata Linn)内生真菌Talaromyces purpureogenus合成银纳米粒子(AgNPs)的方法。Talaromyces purpureoogenus的细胞外提取物显示出次生代谢产物，即萜类和酚类物质。经气相色谱-质谱分析，共鉴定出16种化合物。采用紫外可见光谱、傅里叶变换红外光谱、动态光散射、场发射枪扫描电镜、高分辨率透射电镜、能量色散x射线能谱学和x射线衍射等技术对合成的AgNPs进行表征。紫外可见光谱在380 ~ 470 nm处出现尖峰，表明金属银的存在。FTIR分析显示其含有酚类、羟基和伯胺等多种官能团。在DLS下，合成的AgNPs的z -平均尺寸为240.2 r.nm, PdI为0.720,zeta电位为- 19.6 mV。在fg - sem和HRTEM中，球形AgNPs的直径在30-60 nm之间。在EDS分析中，银的质量百分比为67.26%，原子百分比为43.13%。通过XRD分析发现，真菌合成的AgNPs尺寸为49.3 nm，具有面心立方晶的性质。这些纳米颗粒对单核增生李斯特菌(13±0.29 mm)、大肠杆菌(17±0.14 mm)、痢疾志贺氏菌(18±0.21 mm)和伤寒沙门氏菌(14±0.13 mm)具有显著的抗菌活性。这些合成的AgNPs对2,2′-二苯基-1-苦味酰肼具有有效的自由基清除活性。本研究表明，内生真菌Talaromyces purpureogenus可以作为AgNPs的重要来源，利用生物、生态友好、无毒、抗菌和抗氧化的特性，进一步减轻红豆杉(Taxus baccata)面临的收获压力。图形抽象

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Green synthesis of silver nanoparticles and its antibacterial activity using fungus Talaromyces purpureogenus isolated from Taxus baccata Linn.

The present study is focused on the synthesis of silver nanoparticles (AgNPs) utilizing endophytic fungus Talaromyces purpureogenus, isolated from Taxus baccata Linn. Extracellular extract of Talaromyces purpureogenus has shown occurrence of secondary metabolites viz. terpenoids and phenols. Gas chromatography-mass spectroscopy analysis showed the presence of 16 compounds. Techniques like Ultraviolet–visible spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering, field emission gun scanning electron microscopy, high-resolution transmission electron microscopy, energy-dispersive X-ray spectroscopy and X-ray diffraction were employed to characterize the synthesized AgNPs. UV–Vis spectroscopy showed sharp peaks at 380–470 nm which indicates the presence of metallic silver. FTIR analysis showed the presence of various functional groups like phenols, hydroxyl groups, and primary amines. In DLS, Z-average size and PdI of synthesized AgNPs were 240.2 r.nm and 0.720 respectively, with zeta potential − 19.6 mV. In FEG-SEM and HRTEM the spherical AgNPs showed diameter in the range of 30–60 nm. In EDS analysis the weight percent of Ag is 67.26% and atomic percent is 43.13%. From XRD analysis the size of AgNPs was found to be 49.3 nm with face-centered cubic crystalline nature of fungal synthesized AgNPs. These nanoparticles have shown significant antibacterial activity against tested strains viz. Listeria monocytogenes (13 ± 0.29 mm), Escherichia coli (17 ± 0.14 mm), Shigella dysenteriae (18 ± 0.21 mm) and Salmonella typhi (14 ± 0.13 mm). These synthesized AgNPs have shown effective free radical scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl. The present study showed that the endophytic fungus Talaromyces purpureogenus can be used as a prominent source to synthesize AgNPs by using biological, ecofriendly, and in a non-toxic way accompanied by antibacterial and antioxidant properties which further can reduce the harvesting pressure faced by Taxus baccata.