大颗粒分选分离寄生线虫卵

Q1 Health Professions

Current Protocols in Cytometry Pub Date : 2016-04-01 DOI:10.1002/0471142956.cy1121s76

Alfonso J. Schmidt, T. Bouchery, G. Le Gros, Kylie M. Price

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Large Particle Sorting to Isolate Live Parasitic Nematode Eggs

Traditional jet‐in‐air cell sorters have been designed and optimized to isolate small particles such as mammalian lymphocytes with an average diameter of 10 μm. We discuss the practical considerations of setting up a conventional jet‐in‐air cell sorter, using a 200‐μm nozzle, to isolate the large parasitic nematode eggs of Nippostrongylus brasiliensis, with a maximum size of 60 μm. The eggs were separated based on light scattering properties, no fluorescent dye or molecule was required. © 2016 by John Wiley & Sons, Inc.

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Current Protocols in Cytometry Health Professions-Medical Laboratory Technology

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期刊介绍： Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.