辣木叶的水提取物添加到冷冻填充剂中会破坏山羊精子膜。

IF 1 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2023-05-01
D C de Mello Seal, M Monteiro, L C Pereira Arruda, J H de Souza, R R de Vasconcelos Alves, L A Lira Soares, T H Napoleao, P M Guedes Paiva, P E Bezerra de Lima, R C Bressan Queiroz de Figueiredo, M M Pessoa Guerra
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引用次数: 0

摘要

背景:精液冷冻保存是动物生产中常用的一种生物技术;然而,也存在一些障碍,例如由高水平的活性氧(ROS)引起的障碍。辣木(MO)是抗氧化剂的有效来源,可能是一种重要的佐剂。目的:探讨添加不同浓度MO提取物对山羊精液冷冻保存效果的影响。材料和方法:将来自四个山羊饲养者的Ejaccules(n=6)汇集在脱脂奶(SM)或Tris蛋黄(TEY)基填充剂中稀释,并补充不同浓度的MO提取物(0、1、2和5 mg/mL)。冻融循环后,评估精子动力学和活力。结果:SM延长剂的平直度、摆动度和质膜完整性均低于对照组(P<0.05);TEY延长剂的摆动度低于对照组,5 mg/mL MO提取物的摆动度较对照组低(P<0.05)。结论:在两种稀释液中加入不同浓度的MO叶水提取物,会影响精子进行性参数,并以剂量依赖的方式损害质膜。DOI:10.54680/fr23310110712。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aqueous extract OF Moringa oleifera Lam leaves added to freezing extenders damages goat sperm membranes.

Background: Semen cryopreservation is a biotechnology used frequently in animal production; however, there are some obstacles, such as those caused by high levels of reactive oxygen species (ROS). Moringa oleifera (MO) is known as a potent source of antioxidants and might be an important adjuvant.

Objective: The objective of this study was to determine the effect of different concentrations of MO extract supplementation on goat semen cryopreservation efficiency.

Materials and methods: Ejaculates (n=6) from four goat breeders were pooled and diluted in skimmed milk (SM) or Tris-egg yolk (TEY)-based extenders and supplemented with different concentrations of MO extract (0, 1, 2 and 5 mg/mL). After the freeze-thaw cycle, sperm kinetics and viability were assessed.

Results: With the SM extender, straightness, wobble and plasma membrane integrity were lower than in the control group (P < 0.05). With the TEY extender, wobble was lower in with 5 mg/mL MO extract than in the control group (P < 0.05). As regards sperm ultrastructure, evaluated by SEM, the MO extract, regardless of the diluent used, damaged the membrane of sperm cells in a dose-dependent manner.

Conclusion: The addition of aqueous extract of MO leaves in both diluents at all concentrations tested affects the parameters of sperm progressivity and damages the plasma membrane in a dose-dependent manner. DOI: 10.54680/fr23310110712.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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