分离方案和冷冻保护剂对种马附睾精子冷冻的影响。

IF 1 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2023-05-01
T R Talluri, D Jhamb, N Paul, J Singh, R K Dedar, S C Mehta, R A Legha, Y Pal
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引用次数: 0

摘要

背景:从附睾尾部回收精子可能是从经过阉割或因绞痛或严重损伤突然死亡的优秀种马身上获得遗传物质的唯一选择。目的:评价两种不同方案回收种马附睾精子的效果,并评价不同冷冻保护剂对附睾精子游离性的影响。材料和方法:在全身麻醉下,对3头公马常规去势后立即采集6个附睾。在第一个实验中,使用不同的方法处理同一种马的每个附睾(两个睾丸的)以提取附睾精子,并使用5%甘油或5%二甲基甲酰胺(DMF)作为冷冻保护剂进行合并和冷冻保存。在新鲜、冷冻前和解冻后阶段评估精液质量参数,即进行性动力、HOST、活力和顶体完整性。结果:逆行附睾冲洗法的精液浓度明显高于漂浮法(p<0.05)。与使用5%甘油相比,使用5%DMF作为冷冻保护剂可以显著恢复精液的定性参数,即活力、质膜完整性和顶体完整性。结论:附睾逆行冲洗法的精子浓度明显高于漂浮法,5%DMF作为冷冻保护剂对种马附睾精子具有可接受的游离性。DOI:10.54680/fr23310110312。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of isolation protocols and cryoprotectants on freezing of stallion epididymal spermatozoa.

Background: The recovery of spermatozoa from the cauda epididymis may be the only option to obtain genetic material from elite stallions that had undergone castration or sudden death due to colic or severe injury.

Objective: To evaluate two different protocols for retrieval of stallion epididymal spermatozoa and to evaluate different cryoprotectants on the freezability of the epididymal spermatozoa.

Materials and methods: Six epididymides from three stallions were collected immediately after routine castration under general anesthesia. In the first experiment, each epididymis (of two testes) of the same stallion were processed using different methods for retrieval of the epididymal spermatozoa and were pooled and cryopreserved either using 5% glycerol or 5% dimethyl formamide (DMF) as cryoprotectant. The semen quality parameters viz., progressive motility, HOST, viability and acrosome integrity were evaluated at the fresh, pre-freeze and post-thaw stages.

Results: Retrograde method of flushing of epididymis yielded significantly (p < 0.05) higher concentration of the stallion sperm than that of the floating method. The qualitative semen parameters i.e., viability, plasma membrane integrity and acrosome integrity were found to be significantly restored using 5% DMF as cryoprotectant in comparison to when 5% glycerol was used.

Conclusion: Retrograde flushing method of epididymis yielded significantly higher sperm concentration to that of the floating method, and 5% DMF as cryoprotectant provided acceptable freezability of stallion epididymal spermatozoa. DOI: 10.54680/fr23310110312.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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