MicroRNA-34a-3p及其靶肿瘤坏死因子-α在南印度口腔鳞状细胞癌人群中的调控作用

IF 1.1 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Minerva dental and oral science Pub Date : 2024-10-01 Epub Date: 2023-10-25 DOI:10.23736/S2724-6329.23.04835-0
M S Varshan, Sushmaa C Selvakumar, K A Preethi, Karthikeyan Murthykumar, Dhanraj M Ganapathy, Durairaj Sekar
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引用次数: 0

摘要

背景:最常见的头颈部癌症是口腔鳞状细胞癌(OSCC),这是一种常见的口腔组织学癌症,也是全球最常见的癌症之一。众所周知,已经发现了几种OSCC的生物标志物和治疗靶点,但没有一种对OSCC的进展有效。有趣的是,被称为微小RNA(miRNA)的小型非编码RNA通过靶向许多促进或抑制疾病进展的信号通路或基因来调节细胞活性。令人惊讶的是,在差异表达的miRNA中,miR-34a被鉴定为对OSCC高度敏感和特异,并因其在包括OSCC在内的各种癌症中的作用而被广泛研究。方法:使用生物信息学分析分析miR-34a-3p的二级结构,并使用TargetScan数据库筛选其靶标。从Saveetha牙科学院和医院口腔和颌面部外科收集25例OSCC癌症组织和邻近正常组织的标本。结果:miR-34a-3p的最小自由能为-47.20 kCal,证明了miRNA的稳定性。组织病理学检查证实了OSCC病例,基因表达分析显示miR-34a-3p在OSCC组织中显著下调,而TNF-α的表达则相反。结论:miR-34a-3p可能是OSCC的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MicroRNA-34a-3p and its target tumor necrosis factor-α in the regulation of South Indian oral squamous cell carcinoma population.

Background: The most frequent head and neck cancer is oral squamous cell carcinoma (OSCC), the common histological cancer of the oral cavity and is one of the most prevalent forms of cancer globally. It has been known that there are several biomarkers and therapeutic targets that have been discovered for OSCC, but none of them were effective against the progression of OSCC. Interestingly, small non-coding RNAs termed microRNAs (miRNAs) regulate cellular activity by targeting numerous signaling pathways or genes that either promote or repress the progression of diseases. Surprisingly, among the differentially expressed miRNAs, miR-34a was identified to be highly sensitive and specific to OSCC and widely studied for its role in various cancers, including OSCC.

Methods: The secondary structure of miR-34a-3p was analyzed using bioinformatic analysis and its targets were screened using the TargetScan database. Specimens of 25 OSCC cancer tissues and adjacent normal tissues were collected from the Department of Oral and Maxillofacial Surgery, Saveetha Dental College and Hospitals. The tissues were processed for H&E staining and gene expression analysis of miR-34a-3p and tumor necrosis factor alpha (TNF-α).

Results: The minimum free energy for miR-34a-3p was found to be -47.20 kCal which proved the stability of the miRNA. The histopathological examination confirmed the OSCC cases and the gene expression analysis revealed that miR-34a-3p was significantly downregulated in OSCC tissues, whereas TNF-α showed vice versa expression.

Conclusions: miR-34a-3p could be postulated as a potential therapeutic target for OSCC.

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Minerva dental and oral science
Minerva dental and oral science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.50
自引率
5.00%
发文量
61
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