吡喹莫德诱导的牛皮癣小鼠模型中脊髓小胶质细胞活化的表征

IF 3 3区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of pharmacological sciences Pub Date : 2023-09-20 DOI:10.1016/j.jphs.2023.09.004

Fumihiro Saika , Yohji Fukazawa , Shiroh Kishioka , Norikazu Kiguchi

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引用次数: 0

摘要

尽管小胶质细胞与慢性疼痛有关，但脊髓小胶质细胞在瘙痒调节中的作用尚不清楚。在这项研究中，我们在咪喹莫特（IMQ）诱导的银屑病小鼠模型中表征了脊髓小胶质细胞的激活。局部应用IMQ后，在整个脊髓中观察到肥大（活化）的小胶质细胞。此外，小胶质细胞标志物和炎症介质的mRNA表达上调。使用树脂再生素消融瘙痒相关感觉神经元可减少瘙痒相关的抓挠行为和脊髓背角肥大小胶质细胞的数量。总之，IMQ应用后，感觉神经元输入可能部分有助于脊髓小胶质细胞的激活。

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Characterization of spinal microglial activation in a mouse model of imiquimod-induced psoriasis

Although microglia are associated with chronic pain, the role of spinal microglia in the regulation of itch remains unclear. In this study, we characterized spinal microglial activation in a mouse model of imiquimod (IMQ)-induced psoriasis. Hypertrophic (activated) microglia were observed throughout the spinal cord after the topical application of IMQ. Furthermore, the mRNA expression of microglial markers and inflammatory mediators was upregulated. Ablation of itch-related sensory neurons using resiniferatoxin decreased itch-related scratching behavior and the number of hypertrophic microglia in the spinal dorsal horn. Conclusively, sensory neuron input may partially contribute to spinal microglial activation after IMQ application.

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来源期刊

Journal of pharmacological sciences 医学-药学

CiteScore

6.20

自引率

2.90%

发文量

104

审稿时长

31 days

期刊介绍： Journal of Pharmacological Sciences (JPS) is an international open access journal intended for the advancement of pharmacological sciences in the world. The Journal welcomes submissions in all fields of experimental and clinical pharmacology, including neuroscience, and biochemical, cellular, and molecular pharmacology for publication as Reviews, Full Papers or Short Communications. Short Communications are short research article intended to provide novel and exciting pharmacological findings. Manuscripts concerning descriptive case reports, pharmacokinetic and pharmacodynamic studies without pharmacological mechanism and dose-response determinations are not acceptable and will be rejected without peer review. The ethnopharmacological studies are also out of the scope of this journal. Furthermore, JPS does not publish work on the actions of biological extracts unknown chemical composition.