Azadeh Karimi , Farnaz Kheirandish , Amirreza Javadi Mamaghani , Niloofar Taghipour , Syedeh Fatemeh Mousavi , Ali Aghajani , Nozhat Zebardast , Majid Faraji , Shirzad Fallahi Professor in Medical Parasitology
{"title":"伊朗西部水资源棘阿米巴的鉴定与基因分型","authors":"Azadeh Karimi , Farnaz Kheirandish , Amirreza Javadi Mamaghani , Niloofar Taghipour , Syedeh Fatemeh Mousavi , Ali Aghajani , Nozhat Zebardast , Majid Faraji , Shirzad Fallahi Professor in Medical Parasitology","doi":"10.1016/j.parepi.2023.e00308","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><em>Acanthamoeba</em> spp. is opportunistic amoeba that resides in water, soil, and air. Some pathogenic genotypes of the genus of <em>Acanthamoeba</em> can cause granulomatous amoebic encephalitis (GAE) in people with a defective immune system. The parasite can also cause <em>Acanthamoeba</em> keratitis (AK) among contact lens users. This study was conducted to isolate and identify the <em>Acanthamoeba</em> genotypes in water resources in Lorestan province, western Iran.</p></div><div><h3>Methods</h3><p>Collected 72 water samples from surface and groundwater (springs and aqueducts) in Lorestan province. Samples were filtered and cultured in non-nutrient 1.5% agar medium covered with <em>Escherichia coli (E. coli)</em> at 25 °C. DNA extraction was done and the PCR reaction was performed to detect the <em>Acanthamoeba</em> spp. The positive PCR products were sequenced to determine the genotypes of <em>Acanthamoeba.</em></p></div><div><h3>Results</h3><p>Out of 72 examined water samples, 23.61% were positive for <em>Acanthamoeba</em> sp. by PCR. From PCR-positive samples, 8 (47.05%) samples were T4 genotypes and others were other <em>Acanthamoeba</em> genotypes (T1-T23). Therefore, approximately half of the genotypes belong to the pathogenic T4 genotype.</p></div><div><h3>Conclusions</h3><p>The water examined samples in western provinces of Iran have the potential risk factor for public health. Therefore, the efforts of healthcare providers are needed to identify, train, and prevention from human infections.</p></div>","PeriodicalId":37873,"journal":{"name":"Parasite Epidemiology and Control","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification and genotyping of Acanthamoeba spp. in the water resources of western Iran\",\"authors\":\"Azadeh Karimi , Farnaz Kheirandish , Amirreza Javadi Mamaghani , Niloofar Taghipour , Syedeh Fatemeh Mousavi , Ali Aghajani , Nozhat Zebardast , Majid Faraji , Shirzad Fallahi Professor in Medical Parasitology\",\"doi\":\"10.1016/j.parepi.2023.e00308\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><em>Acanthamoeba</em> spp. is opportunistic amoeba that resides in water, soil, and air. Some pathogenic genotypes of the genus of <em>Acanthamoeba</em> can cause granulomatous amoebic encephalitis (GAE) in people with a defective immune system. The parasite can also cause <em>Acanthamoeba</em> keratitis (AK) among contact lens users. This study was conducted to isolate and identify the <em>Acanthamoeba</em> genotypes in water resources in Lorestan province, western Iran.</p></div><div><h3>Methods</h3><p>Collected 72 water samples from surface and groundwater (springs and aqueducts) in Lorestan province. Samples were filtered and cultured in non-nutrient 1.5% agar medium covered with <em>Escherichia coli (E. coli)</em> at 25 °C. DNA extraction was done and the PCR reaction was performed to detect the <em>Acanthamoeba</em> spp. The positive PCR products were sequenced to determine the genotypes of <em>Acanthamoeba.</em></p></div><div><h3>Results</h3><p>Out of 72 examined water samples, 23.61% were positive for <em>Acanthamoeba</em> sp. by PCR. From PCR-positive samples, 8 (47.05%) samples were T4 genotypes and others were other <em>Acanthamoeba</em> genotypes (T1-T23). Therefore, approximately half of the genotypes belong to the pathogenic T4 genotype.</p></div><div><h3>Conclusions</h3><p>The water examined samples in western provinces of Iran have the potential risk factor for public health. Therefore, the efforts of healthcare providers are needed to identify, train, and prevention from human infections.</p></div>\",\"PeriodicalId\":37873,\"journal\":{\"name\":\"Parasite Epidemiology and Control\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2023-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Parasite Epidemiology and Control\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405673123000259\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasite Epidemiology and Control","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405673123000259","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Identification and genotyping of Acanthamoeba spp. in the water resources of western Iran
Background
Acanthamoeba spp. is opportunistic amoeba that resides in water, soil, and air. Some pathogenic genotypes of the genus of Acanthamoeba can cause granulomatous amoebic encephalitis (GAE) in people with a defective immune system. The parasite can also cause Acanthamoeba keratitis (AK) among contact lens users. This study was conducted to isolate and identify the Acanthamoeba genotypes in water resources in Lorestan province, western Iran.
Methods
Collected 72 water samples from surface and groundwater (springs and aqueducts) in Lorestan province. Samples were filtered and cultured in non-nutrient 1.5% agar medium covered with Escherichia coli (E. coli) at 25 °C. DNA extraction was done and the PCR reaction was performed to detect the Acanthamoeba spp. The positive PCR products were sequenced to determine the genotypes of Acanthamoeba.
Results
Out of 72 examined water samples, 23.61% were positive for Acanthamoeba sp. by PCR. From PCR-positive samples, 8 (47.05%) samples were T4 genotypes and others were other Acanthamoeba genotypes (T1-T23). Therefore, approximately half of the genotypes belong to the pathogenic T4 genotype.
Conclusions
The water examined samples in western provinces of Iran have the potential risk factor for public health. Therefore, the efforts of healthcare providers are needed to identify, train, and prevention from human infections.
期刊介绍：
Parasite Epidemiology and Control is an Open Access journal. There is an increasing amount of research in the parasitology area that analyses the patterns, causes, and effects of health and disease conditions in defined populations. This epidemiology of parasite infectious diseases is predominantly studied in human populations but also spans other major hosts of parasitic infections and as such this journal will have a broad remit. We will focus on the major areas of epidemiological study including disease etiology, disease surveillance, drug resistance and geographical spread and screening, biomonitoring, and comparisons of treatment effects in clinical trials for both human and other animals. We will also look at the epidemiology and control of vector insects. The journal will also cover the use of geographic information systems (Epi-GIS) for epidemiological surveillance which is a rapidly growing area of research in infectious diseases. Molecular epidemiological approaches are also particularly encouraged.
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