Yingying Zhu , Li Shen , Qiuyan Xia , Heyun Tao , Zhanguo Liu , Mengdie Wang , Xiaomin Zhang , Jun Zhang , Jian Lv
{"title":"细胞外囊泡衍生circHIPK3：癌症的新型诊断生物标志物。","authors":"Yingying Zhu , Li Shen , Qiuyan Xia , Heyun Tao , Zhanguo Liu , Mengdie Wang , Xiaomin Zhang , Jun Zhang , Jian Lv","doi":"10.1016/j.advms.2023.10.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Lung cancer (LC) is a common malignancy worldwide. A great number of circular RNAs (circRNAs) have been identified that serve crucial roles in cancer development. Extracellular vesicles (EVs) and their contents have been shown to be biomarkers for the diagnosis and prognosis of LC. Thus, we intended to clarify the functional role of EVs-derived circRNA homology domain interacting protein kinase 3 (EVs-circ<em>HIPK3</em>) and its underlying mechanism of action.</p></div><div><h3>Material and methods</h3><p>Bioinformatics analysis was performed to validate the potential of partially circulating <em>HIPK3</em> in LC diagnosis. EVs were isolated by polyethylene glycol (PEG) precipitation from plasma of 52 LC patients and 30 healthy controls. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to evaluate the expressions of candidate circRNAs (circ<em>HIPK3</em>) and microRNA-637 (miR-637, a target of circ<em>HIPK3</em>).</p></div><div><h3>Results</h3><p>Circ<em>HIPK3</em> is significantly up-regulated in LC, while miR-637 expression is significantly reduced (p < 0.05). Receiver operating characteristic (ROC) curve analysis, based on the expression of EVs-circ<em>HIPK3</em>, allowed us to distinguish LC from healthy controls (area under the curve, AUC 0.897).</p></div><div><h3>Conclusions</h3><p>Taken together, our study shows that EV-derived circ<em>HIPK3</em> can serve as a promising biomarker for LC patient diagnosis. However, the downstream mRNA of the circ<em>HIPK3</em>/miR-637 axis requires further exploration to enrich our understanding of circ<em>HIPK3</em>'s mechanism in LC.</p></div>","PeriodicalId":7347,"journal":{"name":"Advances in medical sciences","volume":"68 2","pages":"Pages 426-432"},"PeriodicalIF":2.5000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Extracellular vesicle-derived circHIPK3: Novel diagnostic biomarker for lung cancer\",\"authors\":\"Yingying Zhu , Li Shen , Qiuyan Xia , Heyun Tao , Zhanguo Liu , Mengdie Wang , Xiaomin Zhang , Jun Zhang , Jian Lv\",\"doi\":\"10.1016/j.advms.2023.10.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Lung cancer (LC) is a common malignancy worldwide. A great number of circular RNAs (circRNAs) have been identified that serve crucial roles in cancer development. Extracellular vesicles (EVs) and their contents have been shown to be biomarkers for the diagnosis and prognosis of LC. Thus, we intended to clarify the functional role of EVs-derived circRNA homology domain interacting protein kinase 3 (EVs-circ<em>HIPK3</em>) and its underlying mechanism of action.</p></div><div><h3>Material and methods</h3><p>Bioinformatics analysis was performed to validate the potential of partially circulating <em>HIPK3</em> in LC diagnosis. EVs were isolated by polyethylene glycol (PEG) precipitation from plasma of 52 LC patients and 30 healthy controls. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to evaluate the expressions of candidate circRNAs (circ<em>HIPK3</em>) and microRNA-637 (miR-637, a target of circ<em>HIPK3</em>).</p></div><div><h3>Results</h3><p>Circ<em>HIPK3</em> is significantly up-regulated in LC, while miR-637 expression is significantly reduced (p < 0.05). Receiver operating characteristic (ROC) curve analysis, based on the expression of EVs-circ<em>HIPK3</em>, allowed us to distinguish LC from healthy controls (area under the curve, AUC 0.897).</p></div><div><h3>Conclusions</h3><p>Taken together, our study shows that EV-derived circ<em>HIPK3</em> can serve as a promising biomarker for LC patient diagnosis. However, the downstream mRNA of the circ<em>HIPK3</em>/miR-637 axis requires further exploration to enrich our understanding of circ<em>HIPK3</em>'s mechanism in LC.</p></div>\",\"PeriodicalId\":7347,\"journal\":{\"name\":\"Advances in medical sciences\",\"volume\":\"68 2\",\"pages\":\"Pages 426-432\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in medical sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1896112623000433\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in medical sciences","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1896112623000433","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Extracellular vesicle-derived circHIPK3: Novel diagnostic biomarker for lung cancer
Purpose
Lung cancer (LC) is a common malignancy worldwide. A great number of circular RNAs (circRNAs) have been identified that serve crucial roles in cancer development. Extracellular vesicles (EVs) and their contents have been shown to be biomarkers for the diagnosis and prognosis of LC. Thus, we intended to clarify the functional role of EVs-derived circRNA homology domain interacting protein kinase 3 (EVs-circHIPK3) and its underlying mechanism of action.
Material and methods
Bioinformatics analysis was performed to validate the potential of partially circulating HIPK3 in LC diagnosis. EVs were isolated by polyethylene glycol (PEG) precipitation from plasma of 52 LC patients and 30 healthy controls. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was employed to evaluate the expressions of candidate circRNAs (circHIPK3) and microRNA-637 (miR-637, a target of circHIPK3).
Results
CircHIPK3 is significantly up-regulated in LC, while miR-637 expression is significantly reduced (p < 0.05). Receiver operating characteristic (ROC) curve analysis, based on the expression of EVs-circHIPK3, allowed us to distinguish LC from healthy controls (area under the curve, AUC 0.897).
Conclusions
Taken together, our study shows that EV-derived circHIPK3 can serve as a promising biomarker for LC patient diagnosis. However, the downstream mRNA of the circHIPK3/miR-637 axis requires further exploration to enrich our understanding of circHIPK3's mechanism in LC.
期刊介绍：
Advances in Medical Sciences is an international, peer-reviewed journal that welcomes original research articles and reviews on current advances in life sciences, preclinical and clinical medicine, and related disciplines.
The Journal’s primary aim is to make every effort to contribute to progress in medical sciences. The strive is to bridge laboratory and clinical settings with cutting edge research findings and new developments.
Advances in Medical Sciences publishes articles which bring novel insights into diagnostic and molecular imaging, offering essential prior knowledge for diagnosis and treatment indispensable in all areas of medical sciences. It also publishes articles on pathological sciences giving foundation knowledge on the overall study of human diseases. Through its publications Advances in Medical Sciences also stresses the importance of pharmaceutical sciences as a rapidly and ever expanding area of research on drug design, development, action and evaluation contributing significantly to a variety of scientific disciplines.
The journal welcomes submissions from the following disciplines:
General and internal medicine,
Cancer research,
Genetics,
Endocrinology,
Gastroenterology,
Cardiology and Cardiovascular Medicine,
Immunology and Allergy,
Pathology and Forensic Medicine,
Cell and molecular Biology,
Haematology,
Biochemistry,
Clinical and Experimental Pathology.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
