使用成年牛血清体外扩增间充质干细胞。

Somia Shehzadi, Maryam Javed, Asmat Ullah, Ahmad Bilal Waqar, Fatima Iftikhar Shah, Sajjad Ullah
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引用次数: 0

摘要

背景:脐带间充质干细胞由于其低免疫原性,越来越多地被用于免疫相关疾病的治疗。然而,UC MSC的原代培养需要在生长培养基中补充血清,由于与细胞培养中常规使用的胎牛血清(FBS)收集方法有关的伦理问题,这带来了挑战。目的:为了解决这一问题,本研究的目的是评估成年牛血清(ABS)作为一种不同且更实惠的血清来源用于体外培养UC MSCs的有效性。用I型胶原酶消化法从出生后立即从奶牛沃顿果冻脐带中分离出UC MSC。方法:从新鲜牛源中收集ABS并热灭活。在倒置显微镜下观察UC MSCs的形态,每两天计算一次生长模式、增殖指数和倍增时间,以比较ABS和FBS的疗效。还对MSC进行了特异性标记物的免疫细胞化学。结果:ABS和FBS在形态、生长速度、种群倍增和增殖指数方面存在显著差异。结论:有趣的是,ABS被证明是体外扩增UC MSCs的生长培养基中的有效补充,为FBS提供了一种可行的替代品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-vitro Augmentation of Mesenchymal Stem Cells by Using Adult Bovine Serum.

Background: Umbilical cord mesenchymal stem cells (UC-MSCs) are increasingly being utilized for immune-related disease therapies due to their low immunogenicity. However, the primary culture of UC-MSCs requires the supplementation of serum in the growth medium, which has posed a challenge due to ethical issues related to the collection method of the fetal bovine serum (FBS) that is routinely used in cell culture.

Aim: In order to address this, the purpose of this research was to assess the effectiveness of adult bovine serum (ABS) as a different and more affordable source of serum for the in-vitro cultivation of UC-MSCs. UC-MSCs were isolated from the umbilical cord of Wharton's jelly of cow immediately after birth, by digestion with Collagenase type I.

Method: ABS was collected from fresh bovine sources and heat-inactivated. The morphology of UC-MSCs was observed under an inverted microscope, and growth patterns, proliferative index, and doubling time were calculated every two days to compare the efficacy of ABS with FBS. Immunocytochemistry for specific markers was also conducted on the MSCs.

Result: The results showed a notable difference in morphology, growth rate, population doubling, and proliferative index between ABS and FBS.

Conclusion: Intriguingly, ABS proved to be an effective supplement in the growth medium for expanding UC-MSCs in vitro, providing a viable alternative to FBS.

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