SFTS患者外周血NK细胞Siglec-9表达的变化可影响NK细胞的功能。

IF 3.3 4区医学 Q3 IMMUNOLOGY

Immunology letters Pub Date : 2023-10-20 DOI:10.1016/j.imlet.2023.10.004

Yaqin Zhang , Ke Jin , Yan Dai , Nannan Hu , Tingting Zhou , Zhan Yang , Ning Ding , Rui Zhang , Ruowei Xu , Jiaying Zhao , Yaping Han , Chuanlong Zhu , Jin Zhu , Jun Li

{"title":"SFTS患者外周血NK细胞Siglec-9表达的变化可影响NK细胞的功能。","authors":"Yaqin Zhang , Ke Jin , Yan Dai , Nannan Hu , Tingting Zhou , Zhan Yang , Ning Ding , Rui Zhang , Ruowei Xu , Jiaying Zhao , Yaping Han , Chuanlong Zhu , Jin Zhu , Jun Li","doi":"10.1016/j.imlet.2023.10.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>To investigate the changes and mechanism of Siglec-9 on NK cells in peripheral blood of patients with severe fever with thrombocytopenia syndrome (SFTS).</p></div><div><h3>Methods</h3><p>First, we used single-cell RNA sequencing to analyze the frequency of NK cells in Peripheral Blood Mononuclear Cells (PBMCs) of SFTS patients and healthy controls (HCs), as well as the differences in the genes on NK cells. Secondly, we analyzed the expression of Siglec-9 and other receptors on NK cells by flow cytometry. Thirdly, we analyzed the correlation between Siglec-9 on NK cells and DBV viral load in plasma.</p></div><div><h3>Results</h3><p>Compared with HCs, the frequency of NK cells in peripheral blood of SFTS patients was significantly decreased, and the activating receptors on NK cells were reduced. The expression of Siglec-9 on NK cells and the frequency of Siglec-9<sup>+</sup>NK cells decreased significantly in SFTS patients. The expression of Siglec-9 on CD16<sup>+</sup>CD56<sup>dim</sup> NK cells was negatively correlated with DBV viral load. In addition, Siglec-9<sup>+</sup>NK cells expressed higher levels of activating receptors and exhibited stronger effector functions than Siglec-9<sup>−</sup>NK cells.</p></div><div><h3>Conclusions</h3><p>The decreased expression of Siglec-9 on NK cells predicts NK cell dysfunction in SFTS patients, suggesting that Siglec-9 may be a potential marker for functional NK cell subsets in SFTS patients.</p></div>","PeriodicalId":13413,"journal":{"name":"Immunology letters","volume":"263 ","pages":"Pages 97-104"},"PeriodicalIF":3.3000,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The change of Siglec-9 expression in peripheral blood NK cells of SFTS patients can affect the function of NK cells\",\"authors\":\"Yaqin Zhang , Ke Jin , Yan Dai , Nannan Hu , Tingting Zhou , Zhan Yang , Ning Ding , Rui Zhang , Ruowei Xu , Jiaying Zhao , Yaping Han , Chuanlong Zhu , Jin Zhu , Jun Li\",\"doi\":\"10.1016/j.imlet.2023.10.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>To investigate the changes and mechanism of Siglec-9 on NK cells in peripheral blood of patients with severe fever with thrombocytopenia syndrome (SFTS).</p></div><div><h3>Methods</h3><p>First, we used single-cell RNA sequencing to analyze the frequency of NK cells in Peripheral Blood Mononuclear Cells (PBMCs) of SFTS patients and healthy controls (HCs), as well as the differences in the genes on NK cells. Secondly, we analyzed the expression of Siglec-9 and other receptors on NK cells by flow cytometry. Thirdly, we analyzed the correlation between Siglec-9 on NK cells and DBV viral load in plasma.</p></div><div><h3>Results</h3><p>Compared with HCs, the frequency of NK cells in peripheral blood of SFTS patients was significantly decreased, and the activating receptors on NK cells were reduced. The expression of Siglec-9 on NK cells and the frequency of Siglec-9<sup>+</sup>NK cells decreased significantly in SFTS patients. The expression of Siglec-9 on CD16<sup>+</sup>CD56<sup>dim</sup> NK cells was negatively correlated with DBV viral load. In addition, Siglec-9<sup>+</sup>NK cells expressed higher levels of activating receptors and exhibited stronger effector functions than Siglec-9<sup>−</sup>NK cells.</p></div><div><h3>Conclusions</h3><p>The decreased expression of Siglec-9 on NK cells predicts NK cell dysfunction in SFTS patients, suggesting that Siglec-9 may be a potential marker for functional NK cell subsets in SFTS patients.</p></div>\",\"PeriodicalId\":13413,\"journal\":{\"name\":\"Immunology letters\",\"volume\":\"263 \",\"pages\":\"Pages 97-104\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-10-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Immunology letters\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0165247823001645\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunology letters","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165247823001645","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

目的：探讨Siglec-9对严重发热伴血小板减少综合征（SFTS）患者外周血NK细胞的影响及其机制。其次，通过流式细胞术分析了Siglec-9等受体在NK细胞上的表达。第三，我们分析了NK细胞上的Siglec-9与血浆中DBV病毒载量的相关性。结果：与HCs相比，SFTS患者外周血NK细胞频率显著降低，NK细胞激活受体减少。在SFTS患者中，Siglec-9在NK细胞上的表达和Siglec-9+NK细胞的频率显著降低。Siglec-9在CD16+CD56dim NK细胞上的表达与DBV病毒载量呈负相关。此外，Siglec-9+NK细胞表达更高水平的激活受体，并表现出比Siglec-9-NK细胞更强的效应器功能。结论：Siglec-9在NK细胞上的表达降低预示着SFTS患者的NK细胞功能障碍，这表明Siglec-九可能是SFTS患者功能性NK细胞亚群的潜在标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The change of Siglec-9 expression in peripheral blood NK cells of SFTS patients can affect the function of NK cells

Objectives

To investigate the changes and mechanism of Siglec-9 on NK cells in peripheral blood of patients with severe fever with thrombocytopenia syndrome (SFTS).

Methods

First, we used single-cell RNA sequencing to analyze the frequency of NK cells in Peripheral Blood Mononuclear Cells (PBMCs) of SFTS patients and healthy controls (HCs), as well as the differences in the genes on NK cells. Secondly, we analyzed the expression of Siglec-9 and other receptors on NK cells by flow cytometry. Thirdly, we analyzed the correlation between Siglec-9 on NK cells and DBV viral load in plasma.

Results

Compared with HCs, the frequency of NK cells in peripheral blood of SFTS patients was significantly decreased, and the activating receptors on NK cells were reduced. The expression of Siglec-9 on NK cells and the frequency of Siglec-9⁺NK cells decreased significantly in SFTS patients. The expression of Siglec-9 on CD16⁺CD56^dim NK cells was negatively correlated with DBV viral load. In addition, Siglec-9⁺NK cells expressed higher levels of activating receptors and exhibited stronger effector functions than Siglec-9⁻NK cells.

Conclusions

The decreased expression of Siglec-9 on NK cells predicts NK cell dysfunction in SFTS patients, suggesting that Siglec-9 may be a potential marker for functional NK cell subsets in SFTS patients.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Immunology letters 医学-免疫学

CiteScore

7.60

自引率

0.00%

发文量

审稿时长

44 days

期刊介绍： Immunology Letters provides a vehicle for the speedy publication of experimental papers, (mini)Reviews and Letters to the Editor addressing all aspects of molecular and cellular immunology. The essential criteria for publication will be clarity, experimental soundness and novelty. Results contradictory to current accepted thinking or ideas divergent from actual dogmas will be considered for publication provided that they are based on solid experimental findings. Preference will be given to papers of immediate importance to other investigators, either by their experimental data, new ideas or new methodology. Scientific correspondence to the Editor-in-Chief related to the published papers may also be accepted provided that they are short and scientifically relevant to the papers mentioned, in order to provide a continuing forum for discussion.