基于碳纳米管/金纳米团簇的FRET荧光检测miRNA

IF 0.9 4区材料科学

Science of Advanced Materials Pub Date : 2023-06-01 DOI:10.1166/sam.2023.4482

Lili Chen, Lingling Chen, Qiuhua Yang, Fengrong Zhang, Q. Qu

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引用次数: 1

摘要

本文提出了一种基于新型FRET对的“开启”荧光分析方法，该方法具有高灵敏度和快速性，用于检测miRNA-195，该对由Lys-Au NCs（通过微波（MW）辅助技术在不到2.5分钟的合成中成功获得）和碳纳米管（CNTs）组成。基于该FRET对，给定0.36 nM的检测限，miRNA-195可以在1–10000 nM的范围内进行定量。此外，利用该方法测量了人血清中miRNA-195的水平，回收率为98.6%-102.4%，表明本文提出的方法适用于实际样品中miRNA-1985的定量检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Carbon Nanotubes/Gold Nanoclusters Based “Turn on” FRET Fluorescent Assay for miRNA Detection

This paper presents a “turn on” fluorescent assay with high sensitivity and rapidity for testing miRNA-195 on the basis of a novel FRET pair consisting of Lys-Au NCs (which have been successfully obtained through less than 2.5 min of synthesis via microwave (MW)-assisted technology) and carbon nanotubes (CNTs). Based on this FRET pair, given a detection limit set at 0.36 nM, miRNA-195 can be quantified in the range of 1–10000 nM. Moreover, the level of miRNA-195 in human serum was measured by virtue of this method, and a recovery of 98.6%–102.4% was achieved, demonstrating that the method put forward in this paper was applicable to actual samples for quantitatively examining miRNA-195.

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来源期刊

Science of Advanced Materials NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY

自引率

11.10%

发文量

审稿时长

4.4 months