由Paenibacillus幼虫诱导的新兴蜂后Apis mellifera L.抗菌基因的评估

IF 1.2 4区农林科学 Q3 ENTOMOLOGY

African Entomology Pub Date : 2022-09-19 DOI:10.17159/2254-8854/2022/a13586

Sas Gomaa, Ems Barakat, M. Salama, EE El Gohary

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引用次数: 0

摘要

本研究评估了用Paenibacillus幼虫（1.07×102CFU/queen）诱导4龄初蜜蜂后，抗微生物abaecin和防御素基因在蜂后中的表达；以研究细菌的存在是否影响免疫反应以及免疫蛋白的基因转录水平。细菌处理后的女王的全身蛋白质显示出高度显著的增加，并且出现了新的蛋白质模式和/或其他蛋白质模式的消失。处理后的女王的基因表达谱显示出相当于防御素基因四倍的调节，而算盘基因没有进展。这一发现同样极大地影响了挑战这种传粉昆虫的疾病。

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Assessment of antibacterial genes of emerging queen Apis mellifera L. induced by the bacterium Paenibacillus larvae larvae

This study evaluated the expression of antimicrobial abaecin and defensin genes in virgin honey-bee queens (Apis mellifera) following induction of 4th larval instar with the bacterium Paenibacillus larvae larvae (1.07 × 102 CFU/queen); to investigate whether the presence of bacteria affects the immune response as well as gene transcript levels for the immune proteins. The total body proteins of bacteria-treated queens showed a highly significant increase, and the appearance of new proteins patterns and/or disappearance of others. The gene expression profile of treated queens showed up-regulation equalled four-fold of the defensin gene, whereas no progression occurred in the abacien gene. This finding likewise greatly affects the diseases challenging this pollinator.

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来源期刊

African Entomology 生物-昆虫学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： African Entomology (ISSN 1021-3589 – print / 2224-8854 – online) replaced the old Journal of the Entomological Society of Southern Africa in 1993. A single volume consisting of two issues (March and September) is published annually. The journal is indexed in all major abstracting journals African Entomology is a peer reviewed scientific journal that publishes original research articles and short communications on all aspects of entomology, with an emphasis on the advancement of entomology on the African continent.