CRISPR‐Cas基因组编辑综述

Current Protocols Essential Laboratory Techniques Pub Date : 2019-11-12 DOI:10.1002/cpet.36

G. Brett Robb

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引用次数: 9

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本文章由计算机程序翻译，如有差异，请以英文原文为准。

Genome Editing with CRISPR-Cas: An Overview

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Genome Editing with CRISPR-Cas: An Overview

The understanding and application of clustered regularly interspaced short palindromic repeat (CRISPR) systems and CRISPR-associated (Cas) nucleases have helped genome editing become a standard laboratory technique. CRISPR-Cas nucleases are RNA-programmed DNA-cutting enzymes that facilitate the introduction of intentional sequence changes into the genomes of experimental cells and organisms. This overview provides a background for genome editing and CRISPR-Cas nucleases, as well as an overview of the technology used to assess the outcomes of genome editing. © 2019 The Authors.

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Current Protocols Essential Laboratory Techniques

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