海洋细菌原Marinomonas primoryensis一种新型孔蛋白的结构特征及电生理特性

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2022-06-16 DOI:10.1134/S1990747822030047

D. K. Chistyulin, E. A. Zelepuga, V. A. Khomenko, O. Yu. Portnyagina, O. D. Novikova

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引用次数: 0

摘要

采用平面双层脂质膜重组蛋白的方法，研究了海洋细菌Marinomonas primoryensis (MpOmp)的新型孔蛋白通道的电生理特性。确定了主要特性:单个MpOmp通道的电导率值，其选择性，以及在各种介质(中性，弱酸性，碱性)中的临界关闭电位值。利用计算机模拟的方法，预测了MpOmp孔的几何特征以及孔口和孔内的电荷分布。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Features of the Structure and Electrophysiological Properties of a Novel Porin from the Marine Bacterium Marinomonas primoryensis

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Features of the Structure and Electrophysiological Properties of a Novel Porin from the Marine Bacterium Marinomonas primoryensis

Using the method of protein reconstitution into planar bilayer lipid membranes, the electrophysiological properties of a novel porin channel from the marine bacterium Marinomonas primoryensis (MpOmp) were characterized. The main characteristics were determined: the conductivity value of the single MpOmp channel, its selectivity, and the values of the critical closing potential in various media (neutral, weakly acidic, alkaline). Using an in silico approach, the geometric characteristics of the MpOmp pore and the distribution of charges at the mouth and inside the porin channel were predicted.

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.