Q. Tang, Yang Xie, Hong Xie, Wenqiang Jiang, Z. Fang
{"title":"七氟醚后处理对离体大鼠心脏缺血再灌注损伤心肌细胞膨胀和自噬的影响","authors":"Q. Tang, Yang Xie, Hong Xie, Wenqiang Jiang, Z. Fang","doi":"10.3329/BJP.V13I3.36437","DOIUrl":null,"url":null,"abstract":"<p>The effects of sevoflurane postconditioning on myocardial oncosis and autophagy were studied in 32 isolated rat heart of ischemia-reperfusion injury. The hearts were perfused by: a) Sham surgery group, b) simple sevoflurane group, c) simple ischemia-reperfusion group (I/R) group, and d) sevoflurane postconditioning group. The ratios between the gray density values of the target bands to the gray density value of GADPH were used to reflect the Beclin-1, LC3II/I, and porimin expression levels. The LC3II/I level in the sevoflurane postconditioning group was lower than the level in the I/R group. The porimin level in the sevoflurane postconditioning group was lower than the level in the I/R group. The myocardial infarction range in the sevoflurane postconditioning group (33 ± 5%) was significantly diminished compared with the range in the I/R group (53 ± 6%) (p<0.05). Sevoflurane decreased the occurrence of oncosis and alleviated myocardial ischemia-reperfusion injury by inhibiting MPTP opening.</p><p><strong>Video Clip of Methodology:</strong></p><p>14 min 31 sec <a href=\"https://www.youtube.com/v/i7dssx_6Jsk\">Full Screen</a> <a href=\"https://www.youtube.com/watch?v=i7dssx_6Jsk\">Alternate</a></p>","PeriodicalId":8719,"journal":{"name":"Bangladesh Journal of Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2018-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/BJP.V13I3.36437","citationCount":"0","resultStr":"{\"title\":\"Effect of sevoflurane postconditioning on myocardial oncosis and autophagy in ischemia-reperfusion injury in isolated rat heart\",\"authors\":\"Q. Tang, Yang Xie, Hong Xie, Wenqiang Jiang, Z. Fang\",\"doi\":\"10.3329/BJP.V13I3.36437\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The effects of sevoflurane postconditioning on myocardial oncosis and autophagy were studied in 32 isolated rat heart of ischemia-reperfusion injury. The hearts were perfused by: a) Sham surgery group, b) simple sevoflurane group, c) simple ischemia-reperfusion group (I/R) group, and d) sevoflurane postconditioning group. The ratios between the gray density values of the target bands to the gray density value of GADPH were used to reflect the Beclin-1, LC3II/I, and porimin expression levels. The LC3II/I level in the sevoflurane postconditioning group was lower than the level in the I/R group. The porimin level in the sevoflurane postconditioning group was lower than the level in the I/R group. The myocardial infarction range in the sevoflurane postconditioning group (33 ± 5%) was significantly diminished compared with the range in the I/R group (53 ± 6%) (p<0.05). Sevoflurane decreased the occurrence of oncosis and alleviated myocardial ischemia-reperfusion injury by inhibiting MPTP opening.</p><p><strong>Video Clip of Methodology:</strong></p><p>14 min 31 sec <a href=\\\"https://www.youtube.com/v/i7dssx_6Jsk\\\">Full Screen</a> <a href=\\\"https://www.youtube.com/watch?v=i7dssx_6Jsk\\\">Alternate</a></p>\",\"PeriodicalId\":8719,\"journal\":{\"name\":\"Bangladesh Journal of Pharmacology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2018-08-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3329/BJP.V13I3.36437\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bangladesh Journal of Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3329/BJP.V13I3.36437\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bangladesh Journal of Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3329/BJP.V13I3.36437","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Effect of sevoflurane postconditioning on myocardial oncosis and autophagy in ischemia-reperfusion injury in isolated rat heart
The effects of sevoflurane postconditioning on myocardial oncosis and autophagy were studied in 32 isolated rat heart of ischemia-reperfusion injury. The hearts were perfused by: a) Sham surgery group, b) simple sevoflurane group, c) simple ischemia-reperfusion group (I/R) group, and d) sevoflurane postconditioning group. The ratios between the gray density values of the target bands to the gray density value of GADPH were used to reflect the Beclin-1, LC3II/I, and porimin expression levels. The LC3II/I level in the sevoflurane postconditioning group was lower than the level in the I/R group. The porimin level in the sevoflurane postconditioning group was lower than the level in the I/R group. The myocardial infarction range in the sevoflurane postconditioning group (33 ± 5%) was significantly diminished compared with the range in the I/R group (53 ± 6%) (p<0.05). Sevoflurane decreased the occurrence of oncosis and alleviated myocardial ischemia-reperfusion injury by inhibiting MPTP opening.
期刊介绍：
Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS).
A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements.
Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review.
Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.
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