香菜乙醇叶提取物及其提取物对大鼠佐剂性关节炎抗氧化作用的比较研究

IF 0.3 4区农林科学 Q4 VETERINARY SCIENCES

Slovenian Veterinary Research Pub Date : 2022-04-22 DOI:10.26873/svr-1150-2022

E. Alum

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引用次数: 5

摘要

摘要：一些研究涉及活性氧在关节炎患者炎症持续和随后关节破坏中的作用。研究了香菜乙醇叶提取物、水溶液和乙酸乙酯组分对类风湿性关节炎雌性wistar白化大鼠氧化应激指数的抗氧化作用。180只大鼠随机分为12组。每组15只大鼠。将没有关节炎的大鼠置于第1组。第2至12组通过向大鼠的左后爪皮内注射0.1ml完全弗氏佐剂来诱导类风湿性关节炎。第2组（关节炎大鼠）没有接受任何治疗，而是给予生理盐水，而第3组（关节病大鼠）接受5mg/kg吲哚美辛TM（标准药物）。第4组至第12组的大鼠以200mg/kg体重、400mg/kg体重和800mg/kg体重的剂量给予样品。弗氏佐剂给药导致炎症和氧化应激，其显著特点是大鼠爪子大小、氧化应激标志物显著增加（P0.05），体重减轻。用标准药物和样品（不同剂量）治疗关节炎大鼠，这导致这些参数的趋势以时间和剂量依赖的方式逆转。接受800mg/kg水性部分的大鼠显示出与吲哚美辛的效果相似的最佳期望结果。因此，马齿苋乙醇提取物和级分可用于治疗氧化应激，氧化应激在类风湿性关节炎患者中非常常见。关键词：类风湿性关节炎；香菜布赫菌；自由基；氧化应激；活性氧物质——香菜乙醇提取物和叶提取物对ALBINA点新鲜辅助关节炎的抗氧化作用：比较研究摘要：活性氧物质在维持骨关节炎患者炎症和随后的关节破坏中的重要性已在几项研究中得到证实。我们研究了香菜叶的乙醇提取物、水溶液和乙酸乙酯部分对患有类风湿性关节炎的雌性白化Wistar大鼠氧化应激指标的抗氧化作用。180只大鼠随机分为12组。每组15只大鼠。将无关节炎的大鼠分为第1组。在第2组至第12组中，治疗因向大鼠左后爪皮内注射0.1ml弗氏完全佐剂而引起的类风湿性关节炎大鼠。治疗初期，第2组（关节炎大鼠）接受生理盐水，第3组（关节病大鼠）给予5 mg/kg吲哚美辛TM（标准药物）。第4至12组的大鼠接受剂量为200、400和800mg/kg体重的佐剂样品。弗氏佐剂的给药引起炎症和氧化应激，这反映在大鼠爪子大小和氧化应激指标的显著增加（p0.05）以及体重的下降。用标准药物和佐剂样品（不同剂量）治疗患有关节炎的大鼠，导致这些参数在时间和剂量方面的趋势逆转。类似于吲哚美辛的最佳结果是在大鼠中接受800mg/kg佐剂水性部分。因此，香菜的乙醇提取物和叶部分可用于管理氧化应激，氧化应激在类风湿性关节炎患者中非常常见。关键词：类风湿性关节炎；香菜布赫菌，；自由基；氧化应激；活性氧类型

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ANTIOXIDANT EFFECT OF Buchholzia coriacea ETHANOL LEAF-EXTRACT AND FRACTIONS ON FREUND’S ADJUVANT-INDUCED ARTHRITIS IN ALBINO RATS: A COMPARATIVE STUDY

Abstract: Several studies have implicated reactive oxygen species in perpetuation of inflammation and subsequent destruction of joints in patients with arthritis. Antioxidant effects of Buchholzia coriacea ethanol leaf-extract, aqueous and ethyl acetate fractions on oxidative stress indices in rheumatoid arthritic female wistar albino rats were examined. 180 rats were randomly divided into 12 groups. Fifteen rats were placed in each group. Rats without arthritis were placed in Group 1. Rheumatoid arthritis was induced in groups 2 to 12 by intradermal injection of 0.1 ml complete Freund’s adjuvant into the left hind paws of rats. Group 2 (arthritic rats) did not receive any treatment but rather were given normal saline while group 3 (arthritic rats) received 5 mg/kg indomethacinTM (a standard drug). Rats in Groups 4 to 12 were administered the samples at doses of 200, 400 and 800 mg/kg body weight. Freund’s adjuvant administration led to inflammation and oxidative stress which were marked by significant (P0.05) increase in paw sizes, oxidative stress markers and reduced body weight of the rats. Arthritic rats were treated with standard drug and samples (at varied doses) and this resulted to reversal of the trend of those parameters in a time and dose-dependent manner. Rats that received 800 mg/kg of the aqueous fraction displayed the best desirable result which was similar to the effect of indomethacin. Thus, Buchholzia coriacea ethanol extract and fractions may be useful in the management of oxidative stress which is very common among individuals with rheumatoid arthritis.Key words: rheumatoid arthritis; Buchholzia coriacea; free radicals; oxidative stress; reactive oxygen speciesANTIOKSIDATIVNI UČINEK ETANOLNEGA IZVLEČKA IN FRAKCIJ LISTOV Buchholzia coriacea NA FREUNDOV ADJUVANTNI ARTRITIS PRI ALBINO PODGANAH: PRIMERJALNA ŠTUDIJAIzvleček: V več študijah je bil nakazan pomen reaktivnih kisikovih zvrsti pri ohranjanju vnetja in poznejšem uničenju sklepov pri pacientih z osteoartritisom. Proučevali smo antioksidantivne učinke etanolovega izvlečka ter vodnih in etil acetatnih frakcij listov Buchholzia coriacea na kazalce oksidativnega stresa pri samicah albino podgan Wistar z revmatoidnih artritisom. 180 podgan smo naključno porazdelili v 12 skupin. V vsaki skupini je bilo 15 podgan. Podgane brez artritisa so bile uvrščene v skupino 1. V skupinah 2 do 12 so bile podgane z revmatoidnim artritisom, povzročenim z intradermalno injekcijo 0,1 ml Freundovega kompletnega adjuvansa v zadnjo levo taco podgan. Skupina 2 (podgane z artritisom) ni bila zdravljena, prejela je fiziološko raztopino, skupina 3 (podgane z artritisom) pa je prejela 5 mg/kg indometacinaTM (standardno zdravilo). Podgane v skupinah 4 do 12 so prejele vzorce adjuvansa v odmerkih 200, 400 in 800 mg/kg telesne mase. Aplikacija Freundovega adjuvansa je povzročila vnetje in oksidativni stres, kar se je kazalo v značilnem (p0.05) povišanju velikosti tac in kazalcev oksidativnega stresa ter zmanjšanju telesne teže podgan. Podgane z artritisom so bile zdravljene s standardnim zdravilom in vzorci adjuvansa (v različnih odmerkih), kar je vodilo v obrat trenda teh parametrov v odvisnosti od časa in odmerka. Najboljši rezultat, podoben učinku indometacina, je bil pri podganah, ki so prejele 800 mg/kg vodne frakcije adjuvansa. Etanolni izvleček in frakcije listov Buchholzia coriacea bi zato lahko bili uporabni pri obvladovanju oksidativnega stresa, ki se zelo pogosto pojavlja pri posameznikih z revmatoidnim artritisom.Ključne besede: revmatoidni artritis; Buchholzia coriacea,; prosti radikali; oksidativni stres; reaktivne kisikove zvrsti

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来源期刊

Slovenian Veterinary Research VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： SLOVENIAN VETERINARY RESEARCH (ISSN 1580-4003) publishes original articles, which report the results of original research in most areas of biomedicine. The journal also publishes review articles dealing with rapidly developing areas of biomedicine or which update understanding of classical fields of biomedicine, as well as case reports, shorter scientific contributions, letters to the editor, etc.; which have not been published or are under consideration for publication elsewhere. Only papers written in English can be considered.