{"title":"假单胞菌对脂肪烃的生物降解","authors":"M. Stancu","doi":"10.17930/agl2022224","DOIUrl":null,"url":null,"abstract":"The present study aimed to evaluate the potential of a Pseudomonas aeruginosa strain to tolerate and degrade aliphatic petroleum hydrocarbons. P. aeruginosa IBB<sub<Ct8</sub< was isolated from a seawater sample using crude oil as the sole carbon source in a minimal medium. The bacterium was identified as P. aeruginosa based on its morphological, biochemical, and molecular characteristics. P. aeruginosa IBB<sub<Ct8</sub<, which possesses alkB (870 bp) and rhlAB (216 bp) genes in its genome, showed a higher tolerance and degradation efficiency of the n-hexadecane, n-decane, cyclohexane, or paraffin oil, compared with the tolerance to n-hexane and its degradation. P. aeruginosa IBB<sub<Ct8</sub< cells produced rhamnolipid biosurfactants which showed a very good emulsification activity against the tested aliphatic hydrocarbons. Due to its characteristics, the isolated bacterium could be used in the bioremediation of petroleum-polluted environments.","PeriodicalId":44979,"journal":{"name":"AgroLife Scientific Journal","volume":" ","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ALIPHATIC HYDROCARBONS BIODEGRADATION BY A Pseudomonas STRAIN\",\"authors\":\"M. Stancu\",\"doi\":\"10.17930/agl2022224\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study aimed to evaluate the potential of a Pseudomonas aeruginosa strain to tolerate and degrade aliphatic petroleum hydrocarbons. P. aeruginosa IBB<sub<Ct8</sub< was isolated from a seawater sample using crude oil as the sole carbon source in a minimal medium. The bacterium was identified as P. aeruginosa based on its morphological, biochemical, and molecular characteristics. P. aeruginosa IBB<sub<Ct8</sub<, which possesses alkB (870 bp) and rhlAB (216 bp) genes in its genome, showed a higher tolerance and degradation efficiency of the n-hexadecane, n-decane, cyclohexane, or paraffin oil, compared with the tolerance to n-hexane and its degradation. P. aeruginosa IBB<sub<Ct8</sub< cells produced rhamnolipid biosurfactants which showed a very good emulsification activity against the tested aliphatic hydrocarbons. Due to its characteristics, the isolated bacterium could be used in the bioremediation of petroleum-polluted environments.\",\"PeriodicalId\":44979,\"journal\":{\"name\":\"AgroLife Scientific Journal\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2022-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"AgroLife Scientific Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17930/agl2022224\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"AgroLife Scientific Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17930/agl2022224","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRONOMY","Score":null,"Total":0}
ALIPHATIC HYDROCARBONS BIODEGRADATION BY A Pseudomonas STRAIN
The present study aimed to evaluate the potential of a Pseudomonas aeruginosa strain to tolerate and degrade aliphatic petroleum hydrocarbons. P. aeruginosa IBB
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