B. Kelln, G. Penner, S. Acharya, T. McAllister, J. Mckinnon, A. Saleem, B. Biligetu, H. Lardner
{"title":"豆类混合物对分批和连续培养(RUSITEC)系统瘤胃发酵、甲烷和微生物产氮的影响","authors":"B. Kelln, G. Penner, S. Acharya, T. McAllister, J. Mckinnon, A. Saleem, B. Biligetu, H. Lardner","doi":"10.1139/cjas-2022-0095","DOIUrl":null,"url":null,"abstract":"The effect of cicer milkvetch (Astragalus cicer L.) and sainfoin (Onobrychis viciifolia Scop.) on ruminal fermentation, methane production, and microbial nitrogen synthesis was assessed in two experiments. Experiment 1 analyzed 2 legumes, cicer milkvetch and sainfoin at 2 stages (vegetative and late flower) incubated with alfalfa (Medicago sativa) at 5 inclusion rates 0:100; 25:75, 50:50, 75:25 and 100:0 (as DM) in batch culture. Experiment 2 analyzed vegetative cicer milkvetch and alfalfa incubated in ratios of 25:75, 50:50, 75:25 and 100:0 (as DM) in continuous culture systems (RUSITEC). In batch culture, increased dry matter disappearance (DMD), and propionate percentage (%, total), and reduced methane (mg g-1 DMD) occurred with vegetative cicer milkvetch inclusion. In RUSITEC, DMD linearly increased (P < 0.01), acetate:propionate ratio quadratically decreased, while ammonia (NH3-N) concentration (P<0.01) and butyrate percentage (% total) linearly decreased (P<0.05) with increasing inclusion of cicer milkvetch. No differences were observed for methane (CH4) production (mg g-1 DMD), or short chain fatty acid (SCFA) production (mmol d-1). Microbial nitrogen synthesis and efficiency of protein synthesis linearly increased (P<0.05) with increased inclusion of cicer milkvetch. Results suggest cicer milkvetch may result in synchronicity of energy and nitrogen during rumen fermentation, which could enhance cattle production.","PeriodicalId":9512,"journal":{"name":"Canadian Journal of Animal Science","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of mixtures of legume species on ruminal fermentation, methane and microbial nitrogen production in batch and continuous culture (RUSITEC) systems\",\"authors\":\"B. Kelln, G. Penner, S. Acharya, T. McAllister, J. Mckinnon, A. Saleem, B. Biligetu, H. Lardner\",\"doi\":\"10.1139/cjas-2022-0095\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The effect of cicer milkvetch (Astragalus cicer L.) and sainfoin (Onobrychis viciifolia Scop.) on ruminal fermentation, methane production, and microbial nitrogen synthesis was assessed in two experiments. Experiment 1 analyzed 2 legumes, cicer milkvetch and sainfoin at 2 stages (vegetative and late flower) incubated with alfalfa (Medicago sativa) at 5 inclusion rates 0:100; 25:75, 50:50, 75:25 and 100:0 (as DM) in batch culture. Experiment 2 analyzed vegetative cicer milkvetch and alfalfa incubated in ratios of 25:75, 50:50, 75:25 and 100:0 (as DM) in continuous culture systems (RUSITEC). In batch culture, increased dry matter disappearance (DMD), and propionate percentage (%, total), and reduced methane (mg g-1 DMD) occurred with vegetative cicer milkvetch inclusion. In RUSITEC, DMD linearly increased (P < 0.01), acetate:propionate ratio quadratically decreased, while ammonia (NH3-N) concentration (P<0.01) and butyrate percentage (% total) linearly decreased (P<0.05) with increasing inclusion of cicer milkvetch. No differences were observed for methane (CH4) production (mg g-1 DMD), or short chain fatty acid (SCFA) production (mmol d-1). Microbial nitrogen synthesis and efficiency of protein synthesis linearly increased (P<0.05) with increased inclusion of cicer milkvetch. Results suggest cicer milkvetch may result in synchronicity of energy and nitrogen during rumen fermentation, which could enhance cattle production.\",\"PeriodicalId\":9512,\"journal\":{\"name\":\"Canadian Journal of Animal Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-07-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Canadian Journal of Animal Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1139/cjas-2022-0095\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Canadian Journal of Animal Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1139/cjas-2022-0095","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Effect of mixtures of legume species on ruminal fermentation, methane and microbial nitrogen production in batch and continuous culture (RUSITEC) systems
The effect of cicer milkvetch (Astragalus cicer L.) and sainfoin (Onobrychis viciifolia Scop.) on ruminal fermentation, methane production, and microbial nitrogen synthesis was assessed in two experiments. Experiment 1 analyzed 2 legumes, cicer milkvetch and sainfoin at 2 stages (vegetative and late flower) incubated with alfalfa (Medicago sativa) at 5 inclusion rates 0:100; 25:75, 50:50, 75:25 and 100:0 (as DM) in batch culture. Experiment 2 analyzed vegetative cicer milkvetch and alfalfa incubated in ratios of 25:75, 50:50, 75:25 and 100:0 (as DM) in continuous culture systems (RUSITEC). In batch culture, increased dry matter disappearance (DMD), and propionate percentage (%, total), and reduced methane (mg g-1 DMD) occurred with vegetative cicer milkvetch inclusion. In RUSITEC, DMD linearly increased (P < 0.01), acetate:propionate ratio quadratically decreased, while ammonia (NH3-N) concentration (P<0.01) and butyrate percentage (% total) linearly decreased (P<0.05) with increasing inclusion of cicer milkvetch. No differences were observed for methane (CH4) production (mg g-1 DMD), or short chain fatty acid (SCFA) production (mmol d-1). Microbial nitrogen synthesis and efficiency of protein synthesis linearly increased (P<0.05) with increased inclusion of cicer milkvetch. Results suggest cicer milkvetch may result in synchronicity of energy and nitrogen during rumen fermentation, which could enhance cattle production.
期刊介绍:
Published since 1957, this quarterly journal contains new research on all aspects of animal agriculture and animal products, including breeding and genetics; cellular and molecular biology; growth and development; meat science; modelling animal systems; physiology and endocrinology; ruminant nutrition; non-ruminant nutrition; and welfare, behaviour, and management. It also publishes reviews, letters to the editor, abstracts of technical papers presented at the annual meeting of the Canadian Society of Animal Science, and occasionally conference proceedings.