摩洛哥毛霉菌溶解磷酸岩能力的定量和定性评估

S. Kribel, S. Qostal, A. Touhami, K. Selmaoui, Afifa Mouria, R. Benkirane, E. Achbani, A. Douira
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引用次数: 1

摘要

从摩洛哥磷矿附近的堆肥、各种作物和土壤中分离出30株木霉分离株,对其溶解磷矿中磷的潜力进行了体外测试。在改良的毕氏琼脂(MPA)固体培养基上对木霉分离株对磷酸盐的增溶作用进行了定性评价。对3天和6天大的培养物的视觉观察没有显示菌落周围有任何明显的区域。然而,所有分离物在孵育3天后都能够在培养基上生长,分离物TR-B 98(3)的最大记录直径为58.6mm,分离物TS-EM-98(2)的最小值为34.8mm。6天后,它们表现出良好的径向生长,超过79.8mm,菌丝密度变化,例如分离株TS-B98、TS-EM-98(1)和TR-CB2000(1),它们分别表现出高、规则和低菌丝密度。此外,木霉分离株在MPA培养基上产生不同数量的分生孢子。对改良Pikovskaya肉汤(MPB)液体培养基的定量估计显示,当不含真菌的液体培养基中可溶性磷的量保持较低时(0.26 mgL–1),木霉分离株溶解磷酸盐的潜力可变。培养9天后,分离株TR-TB 2000的可溶性磷最高浓度为11.92 mgL–1,可溶性磷百分比为95.39%,其次是分离株TR-B 98(3)、TS-B 98和TR-EM 2,分别为11.20、10.47和9.61 mgL–2和89.6、83.76和76.38%,与初始pH(7.2)相比,木霉分离株的最终肉汤pH在TOL分离株的6.81和TS-B-2000的3.40之间变化(2),而在对磷酸盐溶解最弱的分离物如T27中观察到最低的新鲜和干重(FW=1.025g和DW=0.58g)。木霉分离株的高溶解潜力可用于溶解土壤中的固定磷,从而提高土壤肥力和植物生长。分生孢子.mm分离株T1,T2,TS-ML,TS-H,TS-分生孢子3397.02
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantitative and qualitative estimation of MoroccanTrichodermaisolates capacity to solubilize rock phosphate
Thirty Trichoderma isolates isolated from compost, various crops and soil with roots of adjacent sites to the phosphate mines of Morocco, were tested in vitro for their potential to solubilize phosphorus from phosphate rock. The qualitative assessment of phosphate solubilization by Trichoderma isolates was performed on Modified Pikovskaya Agar (MPA) solid medium. The visual observation of the 3- and 6-day-old cultures did not show any clear zone around the colony. However, all the isolates were able to grow on the culture medium 3 days after incubation, the maximum recorded diameter was 58.6 mm for isolate TR-B 98 (3) and the minimum value was 34.8 mm for isolate TS-EM-98 (2). After 6 days, they showed good radial growth that exceeded 79.8 mm with variable appearance of the mycelial density such as the isolates TS-B 98, TS-EM-98 (1) and TR-CB 2000 (1) that presented, respectively, high, regular and low mycelial density. Also, the Trichoderma isolates produced variable number of conidia on MPA medium. Quantitative estimation on the Modified Pikovskaya Broth (MPB) liquid medium showed a variable potential of the Trichoderma isolates to solubilize phosphate when the amount of soluble phosphorus remained low in the liquid medium without the fungus (0.26 mgL –1 ). The maximum concentration of soluble phosphorus was 11.92 mgL –1 with percentage of soluble phosphorus equal to 95.39% recorded by the isolate TR-TB 2000 after 9 days of incubation, followed by the isolates TR-B 98 (3), TS-B 98 and TR-EM 2 respectively, 11.20, 10.47 and 9.61 mgL –1 and 89.6, 83.76 and 76.38%. In addition, treatments with Trichoderma isolates provided a lower final broth pH which varied between 6.81 for TOL isolate and 3.40 for TS-B-2000 (2) compared to initial pH (7.2). The isolates that proved potent for phosphate solubilization displayed the highest fresh and dry weights such as TR-TB 2000 (FW = 4.11 g and DW = 2.56 g), while the lowest fresh and dry weight were noted in the weakest isolates for phosphate solubilization such as T27 (FW = 1.025 g and DW = 0.58 g). The high solubilization potential of Trichoderma isolates can be exploited for the solubilization of fixed phosphorus present in the soil, thus improving soil fertility and plant growth. conidia.mm the isolates T1, T2, TS-ML, TS-H, TS- conidia 3397.02
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来源期刊
Acta Phytopathologica Et Entomologica Hungarica
Acta Phytopathologica Et Entomologica Hungarica Agricultural and Biological Sciences-Plant Science
CiteScore
1.10
自引率
0.00%
发文量
7
期刊介绍: The journal publishes papers on the infectious diseases of plants, damages caused by insects and deals with the basic aspects of chemical and biological protection. Within its field of interest additional topics such as resistance against plant diseases, and physiological, biochemical and molecular questions of plant resistance and susceptibility are also discussed. Publishes book reviews and advertisements.
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