利用Hypnospores作为抗原产生抗olseni Perkinsus单克隆抗体

Q3 Agricultural and Biological Sciences
Sirirat Kaewsalabnil, Anumart Buewkeaw, Janjarus Watanachote, Supannee Leethochavalit, N. Khongchareonporn
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引用次数: 0

摘要

抗体在包括水产养殖在内的许多领域对病原体的检测、鉴定和诊断都很有价值。在这项研究中,我们的目的是产生抗寄生虫Perkinsus olseni的单克隆抗体(mAbs),该抗体是从泰国的波纹蛤中分离出来的。使用斑点印迹和免疫组织化学技术对单克隆抗体进行了表征,以确定其类别及其检测奥氏疟原虫孢子和游动孢子的能力。根据mAb检测到的寄生虫的浓度,所获得的mAb和P.olseni之间的免疫反应产生黑色或灰色斑点。两种反应最强烈的mAb的孢子定量检测阈值为105个细胞/mL(产生灰点)至107个细胞/mL[产生黑点],而游动孢子的定量检测阈值超过107个细胞/mL(灰点)。免疫组织化学分析表明,两种单克隆抗体在感染的蛤蜊组织中的奥氏假单胞菌滋养细胞表面均表现出免疫反应性,表明它们可用于检测和鉴定商业生产的波纹假单胞菌感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti Perkinsus olseni Monoclonal Antibody Generation Using Hypnospores as Antigens
Antibodies are valuable for the detection, identification, and diagnosis of pathogens in many fields, including aquaculture. In this study, we aimed to produce monoclonal antibodies (mAbs) against the parasite Perkinsus olseni, isolated from the clam Paphia undulata in Thailand. The mAbs were characterized using dot blotting and immunohistochemistry techniques in order to identify their class and their ability to detect the hypnospores and zoospores of P. olseni. Immunoreactions between the obtained mAbs and P. olseni produced black or grey spots according to the concentration of parasites detected by the mAbs. The quantitative detection thresholds of hypnospores for the two most strongly reactive mAbs were 105 cells/mL (producing grey spots) to 107 cells/mL (producing black spots), and more than 107 cells/mL (grey spots) for zoospores. Both mAbs showed immunoreactivity at the surface of trophozoite cells of P. olseni in infected clam tissues as assessed by immunohistochemistry analysis, indicating that they could be used for the detection and identification of P. olseni infection in commercial Pa. undulata production.
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来源期刊
Current Applied Science and Technology
Current Applied Science and Technology Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
1.50
自引率
0.00%
发文量
51
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