atOSCA3.1通道的结构

IF 16.8 1区生物学

Nature Structural &Molecular Biology Pub Date : 2018-09-06 DOI:10.2210/PDB5Z1F/PDB

Mingfeng Zhang, Dali Wang, Yunlu Kang, Jing-Xiang Wu, Fuqiang Yao, Chengfang Pan, Zhiqiang Yan, Chen Song, Lei Chen

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引用次数: 13

摘要

机械敏感离子通道将机械刺激转化为离子流。这些通道广泛分布于细菌、高等植物和人类，并参与许多关键的生理过程。在这里，我们发现拟南芥中OSCA蛋白家族的两个成员，即AtOSCA1.1和AtOSCA3.1，属于一类新的机械敏感离子通道。我们通过冷冻电子显微镜求解了分辨率为3.5A的AtOSCA1.1通道和分辨率为4.8A的Atosc3.1通道的结构。OSCA通道是对称的二聚体，由胞质亚单位间相互作用介导。引人注目的是，它们与哺乳动物TMEM16家族蛋白具有结构相似性。我们的结构分析和电生理学研究确定了每个亚基内的离子渗透途径，并提出了激活的构象变化模型。

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Structure of atOSCA3.1 channel

Mechanosensitive ion channels convert mechanical stimuli into a flow of ions. These channels are widely distributed from bacteria to higher plants and humans, and are involved in many crucial physiological processes. Here we show that two members of the OSCA protein family in Arabidopsis thaliana, namely AtOSCA1.1 and AtOSCA3.1, belong to a new class of mechanosensitive ion channels. We solve the structure of the AtOSCA1.1 channel at 3.5-A resolution and AtOSCA3.1 at 4.8-A resolution by cryo-electron microscopy. OSCA channels are symmetric dimers that are mediated by cytosolic inter-subunit interactions. Strikingly, they have structural similarity to the mammalian TMEM16 family proteins. Our structural analysis accompanied with electrophysiological studies identifies the ion permeation pathway within each subunit and suggests a conformational change model for activation.

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来源期刊

Nature Structural &Molecular Biology 生物-生化与分子生物学

自引率

1.80%

发文量

160

期刊介绍： Nature Structural & Molecular Biology is a monthly journal that focuses on the functional and mechanistic understanding of how molecular components in a biological process work together. It serves as an integrated forum for structural and molecular studies. The journal places a strong emphasis on the functional and mechanistic understanding of how molecular components in a biological process work together. Some specific areas of interest include the structure and function of proteins, nucleic acids, and other macromolecules, DNA replication, repair and recombination, transcription, regulation of transcription and translation, protein folding, processing and degradation, signal transduction, and intracellular signaling.