胆汁酸对内陆咸水养殖不同蛋白脂日粮养殖罗非鱼（Oreochromis niloticus）的生长、脂质利用和抗氧化状况的改善

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2023-09-01 DOI:10.1016/j.anifeedsci.2023.115677

Saiprasad Bhusare , Parimal Sardar , Narottam Prasad Sahu , Nazeemashahul Shamna , Pankaj Kumar , Mritunjoy Paul , Prasanta Jana , N. Raghuvaran , U. Bhavatharaniya

{"title":"胆汁酸对内陆咸水养殖不同蛋白脂日粮养殖罗非鱼（Oreochromis niloticus）的生长、脂质利用和抗氧化状况的改善","authors":"Saiprasad Bhusare , Parimal Sardar , Narottam Prasad Sahu , Nazeemashahul Shamna , Pankaj Kumar , Mritunjoy Paul , Prasanta Jana , N. Raghuvaran , U. Bhavatharaniya","doi":"10.1016/j.anifeedsci.2023.115677","DOIUrl":null,"url":null,"abstract":"<div>A 60-day feeding trial was conducted to find out the effect of bile acid supplemented diets with varying protein and lipid levels on growth performance, body composition, antioxidant status and physio-biochemical responses of genetically improved farmed tilapia (GIFT) juveniles reared in inland saline water (ISW) of 10 ppt. Three hetero-nitrogenous (38–32% crude protein, CP), hetero-lipidic (8–14% lipid) and hetero-caloric (409–439 Kcal digestible energy/100 g) diets were supplemented with three levels (0%, 0.05% and 0.1%) of bile acid to prepare nine experimental diets viz. P38L8B0 (38% CP, 8% lipid and 0% bile acid), P38L8B0.05 (38% CP, 8% lipid and 0.05% bile acid), P38L8B0.1 (38% CP, 8% lipid and 0.1% bile acid), P35L11B0 (35% CP, 11% lipid and 0% bile acid), P35L11B0.05 (35% CP, 11% lipid and 0.05% bile acid), P35L11B0.1 (35% CP, 11% lipid and 0.1% bile acid), P32L14B0 (32% CP, 14% lipid and 0% bile acid), P32L14B0.05 (32% CP, 14% lipid and 0.05% bile acid) and P32L14B0.1 (32% CP, 14% lipid and 0.1% bile acid). Four hundred and five acclimated GIFT juveniles (2.51 ± 0.01 g) were randomly distributed in nine treatment groups in triplicate (stocking density 15 fish/tank, 300 L) following 3 × 3 factorial design. Results indicated that body lipid, feed conversation ratio (FCR), amylase and lipase activities, gill and liver superoxide dismutase (SOD) and catalase (CAT) activities and serum glucose (GLU), total protein, triacylglycerol (TAG), total cholesterol (T-CHO), and low density lipoprotein cholesterol (LDL-CHO) were significantly (p < 0.05) increased and weight gain percentage (WG%), specific growth rate (SGR), protease activity and high density lipoprotein cholesterol (HDL-CHO) were significantly (p < 0.05) decreased with increasing dietary lipid levels and more pronounced effect was found in fish fed 32% protein and 14% lipid. Dietary bile acid significantly (p < 0.05) increased WG%, SGR, protein efficiency ratio, lipase activity and HDL-CHO and significantly (p < 0.05) decreased FCR, gill and liver SOD and CAT activities and serum GLU, TAG, T-CHO, and LDL-CHO with more pronounced effect by 0.05% dietary bile acid. In conclusion, feeding of 35% crude protein, 11% lipid and 0.05% bile acid can cause better growth performance, antioxidant status and physio-biochemical responses of GIFT juveniles reared in ISW of 10 ppt. This information will be useful in formulating the bile acid supplemented eco-friendly low protein high lipid (LPHL) feed for profitable culture of GIFT in ISW.</div>","PeriodicalId":7861,"journal":{"name":"Animal Feed Science and Technology","volume":"303 ","pages":"Article 115677"},"PeriodicalIF":2.5000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Bile acid improves growth, lipid utilization and antioxidative status of genetically improved farmed tilapia (Oreochromis niloticus) fed with varying protein-lipid diets reared in inland saline water\",\"authors\":\"Saiprasad Bhusare , Parimal Sardar , Narottam Prasad Sahu , Nazeemashahul Shamna , Pankaj Kumar , Mritunjoy Paul , Prasanta Jana , N. Raghuvaran , U. Bhavatharaniya\",\"doi\":\"10.1016/j.anifeedsci.2023.115677\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>A 60-day feeding trial was conducted to find out the effect of bile acid supplemented diets with varying protein and lipid levels on growth performance, body composition, antioxidant status and physio-biochemical responses of genetically improved farmed tilapia (GIFT) juveniles reared in inland saline water (ISW) of 10 ppt. Three hetero-nitrogenous (38–32% crude protein, CP), hetero-lipidic (8–14% lipid) and hetero-caloric (409–439 Kcal digestible energy/100 g) diets were supplemented with three levels (0%, 0.05% and 0.1%) of bile acid to prepare nine experimental diets viz. P38L8B0 (38% CP, 8% lipid and 0% bile acid), P38L8B0.05 (38% CP, 8% lipid and 0.05% bile acid), P38L8B0.1 (38% CP, 8% lipid and 0.1% bile acid), P35L11B0 (35% CP, 11% lipid and 0% bile acid), P35L11B0.05 (35% CP, 11% lipid and 0.05% bile acid), P35L11B0.1 (35% CP, 11% lipid and 0.1% bile acid), P32L14B0 (32% CP, 14% lipid and 0% bile acid), P32L14B0.05 (32% CP, 14% lipid and 0.05% bile acid) and P32L14B0.1 (32% CP, 14% lipid and 0.1% bile acid). Four hundred and five acclimated GIFT juveniles (2.51 ± 0.01 g) were randomly distributed in nine treatment groups in triplicate (stocking density 15 fish/tank, 300 L) following 3 × 3 factorial design. Results indicated that body lipid, feed conversation ratio (FCR), amylase and lipase activities, gill and liver superoxide dismutase (SOD) and catalase (CAT) activities and serum glucose (GLU), total protein, triacylglycerol (TAG), total cholesterol (T-CHO), and low density lipoprotein cholesterol (LDL-CHO) were significantly (p < 0.05) increased and weight gain percentage (WG%), specific growth rate (SGR), protease activity and high density lipoprotein cholesterol (HDL-CHO) were significantly (p < 0.05) decreased with increasing dietary lipid levels and more pronounced effect was found in fish fed 32% protein and 14% lipid. Dietary bile acid significantly (p < 0.05) increased WG%, SGR, protein efficiency ratio, lipase activity and HDL-CHO and significantly (p < 0.05) decreased FCR, gill and liver SOD and CAT activities and serum GLU, TAG, T-CHO, and LDL-CHO with more pronounced effect by 0.05% dietary bile acid. In conclusion, feeding of 35% crude protein, 11% lipid and 0.05% bile acid can cause better growth performance, antioxidant status and physio-biochemical responses of GIFT juveniles reared in ISW of 10 ppt. This information will be useful in formulating the bile acid supplemented eco-friendly low protein high lipid (LPHL) feed for profitable culture of GIFT in ISW.</div>\",\"PeriodicalId\":7861,\"journal\":{\"name\":\"Animal Feed Science and Technology\",\"volume\":\"303 \",\"pages\":\"Article 115677\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Feed Science and Technology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0377840123001116\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Feed Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0377840123001116","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 1

摘要

本试验旨在研究在10 ppt内陆咸水(ISW)中添加不同蛋白质和脂肪水平的胆汁酸对改良罗非鱼(GIFT)幼鱼生长性能、体成分、抗氧化状态和生理生化反应的影响。在3种不同水平(0%、0.05%和0.1%)的胆汁酸基础上，添加3种不同水平(粗蛋白质38 ~ 32%)、不同水平(脂肪8 ~ 14%)和不同热量(409 ~ 439千卡消化能/100 g)的杂氮饲粮，配制出P38L8B0 (38% CP、8%脂质和0%胆汁酸)、P38L8B0.05 (38% CP、8%脂质和0.05%胆汁酸)、P38L8B0.1 (38% CP、8%脂质和0.1%胆汁酸)、P35L11B0 (35% CP、11%脂质和0%胆汁酸)、P35L11B0.05 (35% CP、11%脂质和0%胆汁酸)、11%脂质和0.05%胆汁酸)、P35L11B0.1 (35% CP、11%脂质和0.1%胆汁酸)、P32L14B0 (32% CP、14%脂质和0%胆汁酸)、P32L14B0.05 (32% CP、14%脂质和0.05%胆汁酸)和P32L14B0.1 (32% CP、14%脂质和0.1%胆汁酸)。试验采用3 × 3因子设计，随机分为9个处理组(放养密度15尾/箱，300 L)，共45尾(2.51±0.01 g)。结果表明，体脂、饲料转化率(FCR)、淀粉酶和脂肪酶活性、鳃和肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性以及血清葡萄糖(GLU)、总蛋白、三酰甘油(TAG)、总胆固醇(T-CHO)和低密度脂蛋白胆固醇(LDL-CHO)均显著高于对照组(p <0.05)、增重率(WG%)、特定生长率(SGR)、蛋白酶活性和高密度脂蛋白胆固醇(HDL-CHO)显著升高(p <0.05)随饲料脂肪水平的升高而降低，其中以32%蛋白质和14%脂肪饲料效果更显著。饲粮胆汁酸显著(p <0.05)显著提高了WG%、SGR、蛋白质效率、脂肪酶活性和HDL-CHO (p <0.05%胆汁酸可显著降低饲料效率、鳃和肝脏SOD和CAT活性以及血清GLU、TAG、T-CHO和LDL-CHO，且效果更为显著。综上所述，10 ppt ISW条件下，35%粗蛋白质、11%脂肪和0.05%胆汁酸能提高GIFT幼鱼的生长性能、抗氧化能力和生理生化反应。这一研究结果将为在ISW养殖GIFT中添加胆汁酸的环保型低蛋白高脂饲料的研制提供参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Bile acid improves growth, lipid utilization and antioxidative status of genetically improved farmed tilapia (Oreochromis niloticus) fed with varying protein-lipid diets reared in inland saline water

A 60-day feeding trial was conducted to find out the effect of bile acid supplemented diets with varying protein and lipid levels on growth performance, body composition, antioxidant status and physio-biochemical responses of genetically improved farmed tilapia (GIFT) juveniles reared in inland saline water (ISW) of 10 ppt. Three hetero-nitrogenous (38–32% crude protein, CP), hetero-lipidic (8–14% lipid) and hetero-caloric (409–439 Kcal digestible energy/100 g) diets were supplemented with three levels (0%, 0.05% and 0.1%) of bile acid to prepare nine experimental diets viz. P₃₈L₈B₀ (38% CP, 8% lipid and 0% bile acid), P₃₈L₈B_0.05 (38% CP, 8% lipid and 0.05% bile acid), P₃₈L₈B_0.1 (38% CP, 8% lipid and 0.1% bile acid), P₃₅L₁₁B₀ (35% CP, 11% lipid and 0% bile acid), P₃₅L₁₁B_0.05 (35% CP, 11% lipid and 0.05% bile acid), P₃₅L₁₁B_0.1 (35% CP, 11% lipid and 0.1% bile acid), P₃₂L₁₄B₀ (32% CP, 14% lipid and 0% bile acid), P₃₂L₁₄B_0.05 (32% CP, 14% lipid and 0.05% bile acid) and P₃₂L₁₄B_0.1 (32% CP, 14% lipid and 0.1% bile acid). Four hundred and five acclimated GIFT juveniles (2.51 ± 0.01 g) were randomly distributed in nine treatment groups in triplicate (stocking density 15 fish/tank, 300 L) following 3 × 3 factorial design. Results indicated that body lipid, feed conversation ratio (FCR), amylase and lipase activities, gill and liver superoxide dismutase (SOD) and catalase (CAT) activities and serum glucose (GLU), total protein, triacylglycerol (TAG), total cholesterol (T-CHO), and low density lipoprotein cholesterol (LDL-CHO) were significantly (p < 0.05) increased and weight gain percentage (WG%), specific growth rate (SGR), protease activity and high density lipoprotein cholesterol (HDL-CHO) were significantly (p < 0.05) decreased with increasing dietary lipid levels and more pronounced effect was found in fish fed 32% protein and 14% lipid. Dietary bile acid significantly (p < 0.05) increased WG%, SGR, protein efficiency ratio, lipase activity and HDL-CHO and significantly (p < 0.05) decreased FCR, gill and liver SOD and CAT activities and serum GLU, TAG, T-CHO, and LDL-CHO with more pronounced effect by 0.05% dietary bile acid. In conclusion, feeding of 35% crude protein, 11% lipid and 0.05% bile acid can cause better growth performance, antioxidant status and physio-biochemical responses of GIFT juveniles reared in ISW of 10 ppt. This information will be useful in formulating the bile acid supplemented eco-friendly low protein high lipid (LPHL) feed for profitable culture of GIFT in ISW.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.