蟾毒灵通过调节MEK/ERK通路抑制人胃癌细胞的生长和上皮向间质转化

IF 0.9 4区医学 Q4 PHARMACOLOGY & PHARMACY

Bangladesh Journal of Pharmacology Pub Date : 2021-01-04 DOI:10.3329/BJP.V16I1.50548

Yi Zhou, Liguo Wang, Hui Lin, Yunxia Wang, Ke-zhu Hou

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引用次数: 2

摘要

本研究旨在评价丁法林对人癌症细胞的抗癌作用，并揭示其潜在机制。结果表明，丁法林能抑制MGC-803胃癌细胞的增殖和集落形成，IC50为10μM。发现这些抗增殖作用是由于诱导G2/M细胞周期停滞所致。G2/M细胞周期阻滞也伴随着对cdc2、cdc25和细胞周期蛋白B1的抑制。此外，bufalin抑制了MGC-803胃癌癌症细胞的上皮-间质转移、迁移和侵袭。Western印迹分析表明，蟾蜍灵是通过去激活EK/ERK信号通路发挥作用的。总之，这些结果表明了丁法林作为癌症化疗发展的先导分子的潜力。

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Bufalin inhibits the growth and epithelial to mesenchymal transition of human gastric cancer cells via modulation of MEK/ERK pathway

This study was designed to evaluate the anti-cancer effects of bufalin against the human gastric cancer cells and unveil the underlying mechanism. The results showed that bufalin inhibited the proliferation and colony formation of the MGC-803 gastric cancer cells and exhibited an IC50 of 10 μM. These antiproliferative effects were found to be due to the induction of G2/M cell cycle arrest. The G2/M cell cycle arrest was also concomitant with inhibition of cdc2, cdc25 and cyclin B1. Furthermore, bufalin suppressed the epithelial-to-mesenchymal transition, migration, and invasion of the MGC-803 gastric cancer cells. The Western blot analysis revealed that bufalin exerted its effects via deactivation of EK/ERK signaling pathway. Taken together, these results suggest the potential of bufalin as the lead molecule for the development of chemotherapy for gastric cancer.

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来源期刊

Bangladesh Journal of Pharmacology PHARMACOLOGY & PHARMACY-

CiteScore

1.50

自引率

18.80%

发文量

审稿时长

>12 weeks

期刊介绍： Bangladesh Journal of Pharmacology (Bangladesh J Pharmacol) is an open access, video component, peer-reviewed journal of the Bangladesh Pharmacological Society (BDPS). A scholarly publication is defined ''open access'' when there are no financial, legal or technical barriers to accessing it. Anyone can read, download, copy, distribute, print or use it in educational purpose within the legal agreements. Peer review of a manuscript means the evaluation of research work by one or more people with similar competences. It can be open or blind. We do the single-blind peer review. Our readers want to know the methodology used by the researchers which is very important to get the results. The video clip of one of the methodologies is prepared by the authors and submit it to publish.