Ellipticine通过对COX-2和NF-κB的双重抑制对卵清蛋白(OVA)诱导的小鼠变应性鼻炎模型的保护作用

IF 0.9 4区 生物学 Q4 PLANT SCIENCES
Jian Wang, Xiao Liu, Zhi Liu, Yanxia Ge, Shu-Jin He
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引用次数: 3

摘要

过敏性鼻炎(AR)是全球范围内一个严重的健康问题。尽管它具有非致命性,但它影响了全世界数百万人,并被认为对影响他们的生活质量和造成重大经济负担负有责任。在目前的研究中,我们旨在使用卵清蛋白(OVA)诱导的过敏性鼻炎小鼠模型来评估椭圆蛋白(ETC)抗AR的抗过敏和抗炎作用以及潜在的分子机制。在OVA致敏后悬浮在5%CMC中,通过腹膜内途径给小鼠施用ETC。研究结果表明,与疾病控制相比,ETC可显著减少鼻子摩擦。与OVA攻击小鼠相比,ETC治疗组的血清组胺、IgG1、TNF-α、IL-1β、MIP-2和IL-6水平显著降低,且呈剂量依赖性。它还能减少AR小鼠BALF中的嗜酸性粒细胞。在蛋白质印迹分析中,与疾病对照小鼠相比,由于TLR-4和胱天蛋白酶-1的抑制,ETC处理组中异常激活的COX-2和NF-ĸB的表达显著降低。ETC与COX-2和NF-ĸB活性位点的残基显示出显著的相互作用。总之,我们的研究结果表明,ETC可用于改善目前针对AR的治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effect of Ellipticine in ovalbumin (OVA)-induced murine model of allergic rhinitis via dual inhibition of COX-2 and NF-κB
Allergic rhinitis (AR) is a serious health concern across the globe. Despite its non-fatal character, it accounts for affecting millions of people across the world and is deemed responsible to affect their quality of life and put a significant economic burden. In the current study, we aimed to assess the anti-allergic and anti-inflammatory effects and the underlying molecular mechanisms of ellipticine (ETC) against AR using ovalbumin (OVA)-induced murine model of allergic rhinitis. The ETC was administered to mice via intra-peritoneal route after suspending in 5% CMC after sensitization by OVA. Results of the study suggested that ETC causes a significant reduction the nose rubs as compared to disease control. A significant reduction in the serum level of histamine, IgG1, TNF-α, IL-1β, MIP-2, and IL-6 was found in ETC treated group in a dose-dependent manner as compared to OVA challenged mice. It also reduces eosinophils in BALF of AR mice. In western blot analysis, the expression of aberrantly activated COX-2 and NF-ĸB found significantly reduced in ETC treated group due to inhibition of TLR-4 and caspase-1 as compared to disease-control mice. ETC showed significant interaction with residues of the active site of COX-2 and NF-ĸB. Collectively, our results indicated that ETC can be used to improve present therapeutic strategies against AR.
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来源期刊
Israel Journal of Plant Sciences
Israel Journal of Plant Sciences 生物-植物科学
CiteScore
1.90
自引率
0.00%
发文量
17
审稿时长
>12 weeks
期刊介绍: The Israel Journal of Plant Sciences is an international journal of extensive scope that publishes special issues dealing with all aspects of plant sciences, including but not limited to: physiology, cell biology, development, botany, genetic
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