同时将甲型流感病毒分型为H1、H3、N1和N2的一锅四链体RT-qPCR检测的综合评价

IF 1.6 Q4 INFECTIOUS DISEASES

Journal of clinical virology plus Pub Date : 2022-11-01 DOI:10.1016/j.jcvp.2022.100119

Hai-Bo Wang, Ji-Can Lin, Hui-Na Huang, Jun-Ting Xu, Cheng-Ning Tu

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引用次数: 0

摘要

本研究建立了一锅四重RT-qPCR检测方法，并进行了综合评价。检测限可低至101拷贝/反应，重复性好，与其他呼吸道病原体无交叉反应。在盲法样本和真实临床样本的临床评估中，该分析与单个商业RT-qPCR分析的符合率为100%。据我们所知，这是第一个用一锅四重RT-qPCR法同时将甲型流感病毒分型为H1、H3、N1和N2的报告，可以提高对未来流感爆发的准备。

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Comprehensive evaluation of an one pot quadruplex RT-qPCR assay for the simultaneously subtyping influenza A virus into H1, H3, N1 and N2

In the current study, an one pot quadruplex RT-qPCR assay was established and comprehensively evaluated. The assay's limit of detection could reach as low as 10¹ copies/reaction, with good repeatability profile and no cross-reaction with other respiratory pathogens. During clinical evaluation both by blinded samples and real clinical samples, the assay exhibited a 100% coincidence rate with individual commercial RT-qPCR assays. To the best of our knowledge, this is the first report on the simultaneous subtyping influenza A virus into H1, H3, N1, and N2 by one pot quadruplex RT-qPCR assay, which could improve the preparedness for future influenza outbreaks.

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来源期刊

Journal of clinical virology plus Infectious Diseases

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

66 days