益生菌干酪乳杆菌对gm - csf佐剂流感DNA疫苗的免疫调节作用

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2022-07-15 DOI:10.2217/fvl-2021-0327

Mehran Mahooti, Elahe Abdolalipour, B. Farahmand, S. Shirian, A. Ghaemi

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引用次数: 0

摘要

目的：研究流感DNA疫苗联合粒细胞-巨噬细胞集落刺激因子（GM-CSF）佐剂和益生菌干酪乳杆菌（口服免疫调节剂）对BALB/c小鼠的保护作用。材料与方法：用HA1DNA疫苗、GM-CSF和益生菌在一周内免疫小鼠两次。结果：两种佐剂在增强DNA疫苗的体液和细胞免疫反应方面具有协同作用。这种组合还降低了肺匀浆中IL-6和IL-17A的水平。保护模式与流感病毒特异性脾细胞增殖和血清IgG抗体（Ab）反应密切相关。结论：干酪乳杆菌对经GM-CSF佐剂的HA1DNA疫苗调节平衡的Th1/Th2免疫应答。

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Immunomodulatory effects of probiotic Lactobacillus casei on GM-CSF-adjuvanted influenza DNA vaccine

Aim: This study investigates the protective efficacy of influenza DNA vaccine combined with a granulocyte macrophage-colony stimulating factor (GM-CSF) adjuvant, and probiotic Lactobacillus casei, an oral immunomodulator, in a BALB/c mice. Materials & methods: The mice were immunized with HA1 DNA vaccine along with GM-CSF and probiotic twice within a one-week interval. Results: The results showed that both adjuvants exert a synergistic effect in enhancing the humoral and cellular immune responses of the DNA vaccine. This combination also deceased IL-6 and IL-17A levels in the lung homogenates. The protection patterns were closely associated with influenza virus-specific splenocyte proliferative and serum IgG antibody (Ab) responses. Conclusion: The Findings demonstrate L. casei modulate balanced Th1/Th2 immune responses toward HA1 DNA vaccine adjuvanted by GM-CSF.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.