针对刺突蛋白K417N和N440K突变的限制性分析检测严重急性呼吸系统综合征冠状病毒2型奥密克戎变异株

IF 0.1 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Investigacion clinica Pub Date : 2022-03-23 DOI:10.54817/ic.v63n1a08

R. Jaspe, J. L. Zambrano, M. Hidalgo, Y. Sulbarán, C. Loureiro, Z. Moros, D. Garzaro, F. Liprandi, H. Rangel, F. Pujol

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引用次数: 4

摘要

到2021年底，南非出现了令人担忧的奥密克戎变异株。这种变体立即引起了人们的关注，因为与它相关的病例爆炸性增加，而且它表现出大量突变。在本研究中，描述了允许检测Spike基因中突变K417N和N440K的限制性位点。这一分析使我们能够提出一种快速识别奥密克戎变异株感染病例的方法。我们表明，所提出的方法有助于提供更多关于这种新型VOC病例的流行率和快速检测的信息。

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Detection of the Omicron variant of SARS-CoV-2 by restriction analysis targeting the mutations K417N and N440K of the spike protein

By the end of 2021, the Omicron variant of concern (VOC) emerges in South Africa. This variant caused immediate concern, due to the explosive increase in cases associated with it and the large number of mutations it exhibits. In this study, the restriction sites that allow detecting the mutations K417N and N440K in the Spike gene are described. This analysis allows us to propose a rapid method for the identification of cases infected with the Omicron variant. We show that the proposed methodology can contribute to provide more information on the prevalence and rapid detection of cases of this new VOC.

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