波多黎各铁线蕨（Pteridae：Vittarioidee）的DNA条形码

IF 0.5 4区生物学 Q4 BIODIVERSITY CONSERVATION

Caribbean Journal of Science Pub Date : 2021-12-20 DOI:10.18475/cjos.v51i2.a13

Karlyn Grafals Nieves, Benjamin W. van Ee

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引用次数: 1

摘要

摘要我们利用叶绿体标记trnL-F和rbcL构建了波多黎各铁线蕨属（Pteridae）的DNA条形码序列库。通过BLAST搜索，trnL-F标记能够成功地确定10个物种中的6个，但有4个失败。对于获得rbcL序列的九个物种，BLAST搜索方法对三个成功，对三个部分成功，三个失败。这两个标记的系统发育分析都能够解析和区分所有采样的分类群，除了宽叶A.latifolium和×vario羽状茎A.vario羽状体的序列被恢复为一个未解决的多体，其中嵌套着海鞘A.petiolatum。分子数据表明，之前在牙买加、古巴、伊斯帕尼奥拉岛上被确定为金字塔A.pyramidale的，根据古巴和波多黎各的数据，波多黎各可能代表了至少两个不同的物种。

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DNA Barcoding of Adiantum (Pteridaceae: Vittarioideae) in Puerto Rico

Abstract We used the chloroplast markers trnL-F and rbcL to construct a DNA barcode sequence library of Adiantum (Pteridaceae) from Puerto Rico. By conducting BLAST searches, the trnL-F marker was able to successfully determine specimens of six out of 10 species for which data were generated, and failed for four. For the nine species for which rbcL sequences were obtained, the BLAST search method was successful for three, partially successful for three, and a failure for three. Phylogenetic analyses of both markers were able to resolve and distinguish all sampled taxa, with the exception that sequences of A. latifolium and A. ×variopinnatum were recovered as an unresolved polytomy with A. petiolatum nested within it. The molecular data indicate that what has previously been determined as A. pyramidale on Jamaica, Cuba, Hispaniola, and Puerto Rico likely represents at least two distinct species, based on data from Cuba and Puerto Rico. We also report the presence of A. ×variopinnatum on Puerto Rico for the first time.

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来源期刊

Caribbean Journal of Science 综合性期刊-生物多样性保护

CiteScore

1.00

自引率

25.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Caribbean Journal of Science publishes articles, research notes, and book reviews pertinent to natural science of the Caribbean region. The emphasis is on botany, zoology, ecology, conservation biology and management, geology, archaeology, and paleontology. The mission as a nonprofit scholarly journal is to publish quality, peer-reviewed papers and to make them widely available.