利用DNA和常染色体单核苷酸多态性进行人类鉴定的最新技术

IF 0.5 Q3 MEDICINE, GENERAL & INTERNAL
F. Khatoon
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引用次数: 2

摘要

法医学中使用的生物样本可能含有由于暴露于多种降解因子中的任何一种而高度碎片化的DNA。聚合酶链式反应产物的序列或大小分析目前负责法医学中人类遗骸的分析。尽管基于PCR的方案是有效的,但模板的拷贝数低以及模板衰减过程带来的变化也存在一定的局限性。本研究的主要目的是通过在犯罪现场对人类进行鉴定,探讨常染色体SNPs在法医学中的意义。本研究探索了常染色体SNPs在犯罪现场人类识别中的适用性。这将填补目前文献中关于常染色体SNPs在犯罪现场调查中识别人类的意义的空白。它还将能够识别参与从一般盗窃到强奸和性侵犯、谋杀和抢劫等几类犯罪的罪犯。它还将允许在由于尸体无法辨认而难以辨认死者的情况下辨认尸体。这项研究将有助于改进犯罪现场调查员的调查工作。它将为将分子遗传学的最新技术纳入法医学提供一条重要的途径。通过将这项研究的成果落实到法医学领域,也将减少犯罪现场调查员的工作量。有几项研究证明了SNPs在法医调查中用于识别犯罪现场的人类的适用性。研究人员开发了几种有效的技术系统,能够对含有降解DNA的生物样品进行分析,因为可以从这些样品中获得SNPs。个体的身体特征可以通过SNPs的分析来预测。这可以提供有关犯罪人员眼睛、头发和皮肤颜色的重要信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
RECENT TECHNIQUES BASED ON THE UTILIZATION OF DNA AND AUTOSOMAL SINGLE NUCLEOTIDE POLYMORPHISMS FOR IDENTIFYING HUMANS
The biological samples used in forensics can contain DNA which is highly fragmented as a consequence of exposure to any of the numerous degrading factors. Analysis of the sequence or size of the products of Polymerase chain reaction is at present responsible for the analysis of remains of humans in forensics. Despite the effectiveness of protocols based on PCR, there are certain limitations that are presented by the low numbers of copies of the template and the variations that are imposed by the decaying process to the template. The primary aim of this research is to explore the significance of autosomal SNPs in forensic science through the identification of humans at a crime scene. This study provides an exploration of the applicability of autosomal SNPs for the identification of humans at crime scene. This would fill the gap present in the current literature regarding the significance of autosomal SNPs in the identification of humans during crime scene investigation. It will also enable the identification of the criminals involved in several types of the crimes ranging from general theft to rape and sexual assault, murder, and robberies. It will also allow the identification of dead bodies in cases where it is difficult to identify the dead person due to unrecognizable condition of the body. This study will facilitate the improvement of the investigation of crime scene investigators. It will provide a significant way for the incorporation of recent techniques of the molecular genetics into forensics. Reduction in the workload of the crime scene investigators would also occur through the implementation of outcomes of this study into the field of forensic science. There are several studies which have demonstrated the applicability of SNPs in forensic investigations for identifying the humans at crime scene. Several effective and efficient technological systems have been developed by the researchers which are capable of performing analysis of biological samples containing degraded DNA because SNPs can be obtained from these samples. Physical characteristics of the individuals can be predicted through the analysis of SNPs. This can provide significant information about the color of eye, hair and skin of the individuals involved in crime.
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来源期刊
Gomal Journal of Medical Sciences
Gomal Journal of Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
0.60
自引率
80.00%
发文量
37
审稿时长
40 weeks
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