phiC31整合酶系统在干细胞生物学与技术中的应用综述

Q1 Biochemistry, Genetics and Molecular Biology

Frontiers in Life Science Pub Date : 2018-01-01 DOI:10.1080/21553769.2018.1447516

Adham Fani Maleki, M. Sekhavati

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引用次数: 1

摘要

干细胞的两大特性:自我更新和分化能力，使其在广泛的科学领域，特别是干细胞的分子和细胞生物学以及再生医学中占有重要地位。基因靶向在制造修饰干细胞中起着关键作用，这被认为是再生医学的支柱。phiC31整合酶系统作为一种位点特异性重组酶，可能是实现这一目标的一种简单有效的方法。鉴于phiC31整合酶系统在干细胞中的应用潜力巨大，本文综述了前人的研究成果，重点对干细胞类型及其应用进行了综述。

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Application of phiC31 integrase system in stem cells biology and technology: a review

ABSTRACT Two specific characteristics of stem cells, self-renewal and their differentiation capacity have made them important for vast scientific areas, particularly in the molecular and cell biology of stem cells, as well as regenerative medicine. Gene targeting plays a pivotal role in creating modified stem cells, which are considered the mainstay in regenerative medicine. The phiC31 integrase system, as a site-specific recombinase, could be a straightforward and efficient approach for this aim. According to the high potential of the application of phiC31 integrase system in stem cells, the present paper reviews previous studies, placing the focus on stem cell types and their application.

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来源期刊

Frontiers in Life Science MULTIDISCIPLINARY SCIENCES-

CiteScore

5.50

自引率

0.00%

发文量

期刊介绍： Frontiers in Life Science publishes high quality and innovative research at the frontier of biology with an emphasis on interdisciplinary research. We particularly encourage manuscripts that lie at the interface of the life sciences and either the more quantitative sciences (including chemistry, physics, mathematics, and informatics) or the social sciences (philosophy, anthropology, sociology and epistemology). We believe that these various disciplines can all contribute to biological research and provide original insights to the most recurrent questions.