一种新的低温方法3D打印细胞兼容,导电,水凝胶基油墨

IF 2.3 4区 工程技术 Q3 ENGINEERING, MANUFACTURING
3D Printing and Additive Manufacturing Pub Date : 2024-04-01 Epub Date: 2024-04-16 DOI:10.1089/3dp.2022.0169
Aida Shoushtari Zadeh Naseri, Cormac Fay, Andrew Nattestad, Gregory Ryder, Sepidar Sayyar, Zhilian Yue, Xiao Liu, David L Officer, Gordon G Wallace
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引用次数: 0

摘要

在组织工程和再生医学领域,开发模仿原生细胞外基质的细胞相容性三维导电支架对可兴奋细胞和组织工程至关重要。本研究开发了一种定制的低温挤压三维打印机,可控制油墨和打印表面的温度。利用这种方法,水性油墨被高精度地打印成定义明确的层。利用壳聚糖(CS)和边缘功能化膨胀石墨烯(EFXG)开发出了导电水凝胶墨水。对不同的 EFXG:CS 比例(60:40 和 80:20)进行了评估,以确定导电性和可印刷性。使用新型定制低温三维打印机,可打印出 2 到 20 层的导电结构,特征尺寸小至 200 μm。打印出的结构具有机械坚固性和导电性。水合状态下的最高杨氏模量和导电率分别为 2.6 兆帕和∼45 S/m。细胞相容性实验表明,所开发的材料可支持 NSC-34 小鼠运动神经元样细胞的存活、附着和增殖。三维打印结构独特的机械和电学特性使其成为三维结构可兴奋细胞工程的理想候选材料。此外,这种新型打印装置还可用于高精度、高分辨率地打印其他基于水凝胶的墨水。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Novel Cryogenic Approach to 3D Printing Cytocompatible, Conductive, Hydrogel-Based Inks.

In the field of tissue engineering and regenerative medicine, developing cytocompatible 3D conductive scaffolds that mimic the native extracellular matrix is crucial for the engineering of excitable cells and tissues. In this study, a custom cryogenic extrusion 3D printer was developed, which afforded control over both the ink and printing surface temperatures. Using this approach, aqueous inks were printed into well-defined layers with high precision. A conductive hydrogel ink was developed from chitosan (CS) and edge-functionalised expanded graphene (EFXG). Different EFXG:CS ratios (between 60:40 and 80:20) were evaluated to determine both conductivity and printability. Using the novel customized cryogenic 3D printer, conductive structures of between 2 and 20 layers were produced, with feature sizes as small as 200 μm. The printed structures are mechanically robust and are electrically conducting. The highest Young's modulus and conductivity in a hydrated state were 2.6 MPa and ∼45 S/m, respectively. Cytocompatibility experiments reveal that the developed material supports NSC-34 mouse motor neuron-like cells in terms of viability, attachment, and proliferation. The distinctive mechanical and electrical properties of the 3D-printed structures would make them good candidates for the engineering of 3D-structured excitable cells. Moreover, this novel printing setup can be used to print other hydrogel-based inks with high precision and resolution.

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来源期刊
3D Printing and Additive Manufacturing
3D Printing and Additive Manufacturing Materials Science-Materials Science (miscellaneous)
CiteScore
6.00
自引率
6.50%
发文量
126
期刊介绍: 3D Printing and Additive Manufacturing is a peer-reviewed journal that provides a forum for world-class research in additive manufacturing and related technologies. The Journal explores emerging challenges and opportunities ranging from new developments of processes and materials, to new simulation and design tools, and informative applications and case studies. Novel applications in new areas, such as medicine, education, bio-printing, food printing, art and architecture, are also encouraged. The Journal addresses the important questions surrounding this powerful and growing field, including issues in policy and law, intellectual property, data standards, safety and liability, environmental impact, social, economic, and humanitarian implications, and emerging business models at the industrial and consumer scales.
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