牛蒡果实提取物对雌性Wistar大鼠的急性和反复口服毒性研究

IF 1.1 Q4 PHARMACOLOGY & PHARMACY
M. Yaghoubi, Z. Mousavi, T. Rastegar, G. Amin
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引用次数: 5

摘要

背景与目的菊科菊芋(Arctium lappa)是一种药用和营养补充剂。这种植物的果实、叶子和根部以其药用作用而闻名。本研究对果实提取物对雌性大鼠的毒性进行了研究。方法采用大鼠灌胃法建立牛蒡果实提取物急性和重复给药模型,评价其毒性。将动物分为两组:对照组和试验组。在急性毒性模型中,对动物给予1000和5000 mg/kg ALFE。观察了14天的中毒症状、体重、死亡和异常行为。在重复毒性模型中,每天给予ALFE(300mg/kg),持续4周。评估生化和组织病理学变化,并与对照组进行比较。通过单向方差分析确定统计学显著性,然后使用GraphPad Prism 6进行Tukey检验。结果急性试验中没有发现死亡率;因此,在雌性大鼠中测定的口服LD50值大于5000mg/kg。在重复试验中,动物接受ALFE(300mg/kg),没有观察到死亡。血液学和血清化学参数没有显示出统计学上显著的变化。组织病理学研究显示,肺和小肠两个主要器官存在显微镜下病变。结论ALFE对大鼠经口急性毒性较低,LD50大于5000mg/kg。此外,当以300 mg/kg的剂量亚慢性给药时,他们发现几个器官有轻微的组织损伤。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Safety Assessment of Arctium lappa L. Fruit Extract in Female Wistar Rats: Acute and Repeated Oral Toxicity Studies
Background and objectivesArctium lappa belonging to the Compositae (Asteraceae) family has been used as a medicinal and nutritional supplement in the world. The fruits, leaves and roots of the plant are well-known for their pharmaceutical effects. Toxicity of the fruit’s extract in female rats was investigated in the present study.MethodsTo assess the toxicity profile of Arctium lappa fruit extract (ALFE), it was administered to rats by gavage in acute and repeated models. The animals were divided into two groups: control and test groups. In the acute toxicity model, 1000 and 5000 mg/kg ALFE were administered to the animals. Toxic symptoms, body weight, death and abnormal behaviors were observed for 14 days. In the repeated toxicity model, ALFE (300 mg/kg) was daily administered for 4 weeks. Biochemical and histopathological changes were assessed and compared with the control group. Statistical significance was determined by one-way analyses of variance, followed by the Tukey test using GraphPad Prism 6.ResultsNo mortality was noticed in the acute test; therefore, the oral LD50 value determined in the female rats was greater than 5000 mg/kg. In the repeated test, the animals received ALFE (300 mg/kg) and no mortality was observed. The hematology and serum chemistry parameters showed no statistically significant changes.  The histopathological studies revealed evidences of microscopic lesions in two main organs lungs and small intestine.ConclusionThe results indicated that the oral acute toxicity of ALFE in the rats was of a low order with LD50 being more than 5000 mg/kg. Moreover, they revealed slight tissue damage to several organs when sub-chronically administered at a dose of 300 mg/kg.
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来源期刊
Research Journal of Pharmacognosy
Research Journal of Pharmacognosy PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
20.00%
发文量
0
审稿时长
8 weeks
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