超稀释泽兰通过调节TGFβ1、MMP-9和干扰素减轻DENV诱导的纤维化

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-09-07 DOI:10.2217/fvl-2023-0121

Avipsha Sarkar, Anirban Roy, Madhulina Maity, D. Nayak, Satadal Das

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引用次数: 0

摘要

目的：本研究旨在用超稀释的泽兰（UEP）在鸡胚模型中减轻登革病毒（DENV）介导的纤维化。材料与方法：对感染的鸡胚绒毛尿囊膜和肝脏进行DENV发病机制的检测。用MTT法和划痕法测定UEP的细胞毒性。DENV感染后的细胞病变试验在体外进行，以检查UEP及其成分的作用。组织病理学分析显示DENV介导的纤维化和UEP的作用。qRT-PCR和明胶酶谱显示纤维化引起基因的水平。结果：UEP通过调节纤维化生物标志物TGF-β、MMP-1、MMP-9、TIMP-1和IFNs，在体内减轻DENV介导的纤维化方面表现出低细胞毒性和最大潜力。结论：UEP可减轻DENV介导的发病机制。

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Ultradiluted Eupatorium perfoliatum alleviates DENV-induced fibrosis by regulation of TGFβ1, MMP-9 and interferons

Aim: This study aimed to alleviate dengue virus (DENV)-mediated fibrosis in a chick embryo model using ultradiluted Eupatorium perfoliatum (UEP). Materials & methods: Chorioallantoic membrane and liver of infected embryonated chicken eggs were checked for DENV pathogenesis. Cytotoxicity of UEP was assessed by MTT and scratch assay. Cytopathic assay after DENV infection was performed in vitro to check the effect of UEP and its constituents. Histopathology analysis revealed DENV-mediated fibrosis and the effect of UEP. qRT-PCR and gelatin zymography showed levels of fibrosis-causing genes. Results: UEP showed low cytotoxicity and maximum potential in mitigating DENV-mediated fibrosis in vivo via regulation of fibrosis biomarkers TGF-β, MMP-1, MMP-9, TIMP-1 and IFNs. Conclusion: UEP may be considered to alleviate DENV-mediated pathogenesis.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.