腹泻绵羊空肠弯曲杆菌分离株的耐药性和毒力基因

Q4 Veterinary

World''s Veterinary Journal Pub Date : 2022-06-25 DOI:10.54203/scil.2022.wvj24

A. Hafez

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引用次数: 1

摘要

空肠弯曲杆菌是世界范围内引起肠胃炎的重要病原体之一。本研究旨在检测从绵羊粪便中获得的空肠弯曲菌中的五个毒力基因（flaA、virB11、ciaB、iam和dnaJ）和两个抗生素抗性基因（gyrA和tetO）。用聚合酶链式反应检测了64株空肠弯曲菌的毒力基因。用微量稀释法筛选对5种抗生素（环丙沙星、红霉素、庆大霉素、链霉素和四环素）的表型耐药性。使用特异性引物通过PCR检测具有抗体图谱的分离株的gyrA和tetO基因。毒力基因flaA（32%）和dnaJ（29%）的患病率最高。测试的空肠弯曲菌分离株对喹诺酮类药物（68.3%）和四环素类药物（48.4%）均表现出高耐药性，gyrA和tetO基因的抗生素耐药性患病率增加。庆大霉素和红霉素为治疗弯曲杆菌病提供了更好的替代药物。为了推广这一发现，需要对弯曲杆菌的几种菌株进行涉及更多毒力基因的广泛分析。

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Antimicrobial Resistance and Virulence Genes of Campylobacter jejuni Isolates from Diarrheic Sheep

One of the important agents causing gastroenteritis worldwide is Campylobacter jejuni (C. jejuni). The current study aimed to detect five virulence genes (flaA, virB11, ciaB, iam, and dnaJ) and two antibiotic resistance genes (gyrA and tetO) in C. jejuni obtained from sheep stool. The virulence genes were detected by PCR in 64 C. jejuni strains. The phenotypic resistance to five selected antibiotics (Ciprofloxacin, Erythromycin, Gentamycin, Streptomycin, and Tetracycline) was screened with the microdilution method. The isolates with antibiograms were tested for detection of gyrA and tetO genes via PCR using specific primers. The virulence genes flaA (32%) and dnaJ (29%) had the highest prevalence. The tested isolates of C. jejuni revealed high resistance to both quinolone (68.3%) and tetracycline groups (48.4%) with an increased prevalence of antibiotic resistance of gyrA and tetO genes. Gentamycin and erythromycin offered better alternative drugs for the treatment of campylobacteriosis. To generalize the findings, extensive profiling that involves more virulence genes is required in several strains of Campylobacter.

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来源期刊

World''s Veterinary Journal Veterinary-Veterinary (all)

CiteScore

1.00

自引率

0.00%

发文量

期刊介绍： The World''s Veterinary Journal (ISSN 2322-4568) is an international, peer reviewed open access journal aims to publish the high quality material from veterinary scientists'' studies. All accepted articles are published Quarterly in full text on the Internet. WVJ publishes the results of original scientific researches, reviews, case reports and short communications, in all fields of veterinary science. In details, topics are: Behavior Environment and welfare Animal reproduction and production Parasitology Endocrinology Microbiology Immunology Pathology Pharmacology Epidemiology Molecular biology Immunogenetics Surgery Virology Physiology Vaccination Gynecology Exotic animals Animal diseases Radiology Ophthalmology Dermatology Chronic disease Anatomy Non-surgical pathology issues of small to large animals Cardiology and oncology.