自噬相关基因16L1在慢性斑块型银屑病中的价值

IF 0.2 Q4 DERMATOLOGY
Rehab Naguib, Abd-ElAziz El-Rifaie, EmanA.Z. Eissa, L. Rashed
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引用次数: 0

摘要

背景银屑病是一种慢性高增殖性炎症性疾病,遗传和环境因素在其中起着重要作用,但其确切病因尚不清楚。自噬是一种严格调控的溶酶体降解途径,对维持细胞内稳态和正常发育至关重要。自噬相关基因的失调已被认为会增加对疾病的易感性,如炎症、癌症和自身免疫性疾病。目的本研究旨在检测自噬相关基因16L1(ATG 16L)在银屑病患者中的表达,并与正常对照组进行比较,以探讨自噬在银屑病发病机制中的作用。患者和方法本病例对照研究包括30名银屑病患者和30名健康对照。穿孔皮肤活检4 mm从银屑病病变中提取,然后从对照中提取,为了研究参数的稳定性,将其保存在裂解溶液中,并在-80°C下冷冻,直到使用实时PCR分析ATG 16L。结果银屑病病变皮肤ATG16L1水平较正常对照组明显升高(P<0.001),本研究纳入的患者数量有限(30例)。结论自噬过程可能在银屑病的发病机制中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Autophagy-related gene 16L1 value in chronic plaque psoriasis
Background Psoriasis is a chronic hyperproliferative inflammatory disease, in which genetic and environmental factors have an important role, but the exact cause is yet unknown. Autophagy is a strictly regulated lysosomal degradation pathway that is crucial for maintaining intracellular homeostasis and normal development. Dysregulation of autophagy-related genes has been recognized to increase susceptibility to diseases, such as inflammation, cancer, and autoimmune disorders. Aim Our study aimed to detect the expression of the autophagy-related gene 16L1 (ATG 16L) in psoriasis patients compared with normal controls to investigate the role of autophagy in the pathogenesis of this disease. Patients and methods This case–control study included 30 psoriasis patients and 30 healthy controls. Punch skin biopsies of 4 mm were taken from psoriatic lesions and then from the controls and they were kept in a lysis solution for the stability of the studied parameters and were kept frozen at –80°C till analysis of ATG 16L using real-time PCR. Results The level of the ATG 16L1 in the lesional skin of psoriasis was significantly increased compared with normal controls (P<0.001). Limitation Limited number of patients were included in this study (30 patients). Conclusion Autophagy process may play an important role in the pathogenesis of psoriasis disease.
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