SOD1-Tg小鼠脊髓组织病理学变化及运动神经元动力学

IF 0.9 4区医学 Q4 PATHOLOGY

Journal of Toxicologic Pathology Pub Date : 2021-10-01 DOI:10.1293/tox.2021-0056

Masaharu Tanaka, Kengo Homma, A. Soejima

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引用次数: 1

摘要

我们分析了经常用作肌萎缩侧索硬化症（ALS）疾病模型的Cu/Zn超氧化物歧化酶转基因（SOD1G93ATg）小鼠腰脊髓的组织病理学变化和运动神经元（MNs）的数量。在SOD1G93ATg小鼠中，在出现神经症状之前的7周龄时，在神经元细胞体中观察到透明内含物和泡沫状液泡，并且在13周龄后，大液泡、球体形成和神经细胞聚集变得突出。健康MN的数量在野生型小鼠中为28.7至37.1个细胞/动物，在转基因（Tg）小鼠中为9.3至13.6个细胞/小鼠。此外，Tg小鼠的MN数量，包括退行性神经元，在18周龄时为27.3–36.1个细胞/只，在21周龄为17.8–19.6个细胞/两只。目前的研究结果为ALS治疗药物的开发提供了有用的信息。

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Histopathological changes of the spinal cord and motor neuron dynamics in SOD1 Tg mice

We analyzed the histopathological changes and the number of motor neurons (MNs) in the lumbar spinal cord of Cu/Zn superoxide dismutase transgenic (SOD1G93ATg) mice, which are frequently used as a disease model of amyotrophic lateral sclerosis (ALS). In SOD1G93ATg mice, hyaline inclusions and foamy vacuoles in the neuronal cell body were observed at 7 weeks of age before neurologic symptoms, and large vacuoles, spheroid formation, and nerve cell aggregation became prominent after 13 weeks of age. The number of healthy MNs was 28.7 to 37.1 cells/animal in wild-type mice and 9.3 to 13.6 cells/animal in transgenic (Tg) mice. Furthermore, the number of MNs, including degenerative neurons, in Tg mice was 27.3–36.1 cells/animal at 18 weeks of age and 17.8–19.6 cells/animal at 21 weeks of age. The present results provide useful information for the development of drugs in ALS treatment.

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来源期刊

Journal of Toxicologic Pathology PATHOLOGY-TOXICOLOGY

CiteScore

2.10

自引率

16.70%

发文量

审稿时长

>12 weeks

期刊介绍： JTP is a scientific journal that publishes original studies in the field of toxicological pathology and in a wide variety of other related fields. The main scope of the journal is listed below. Administrative Opinions of Policymakers and Regulatory Agencies Adverse Events Carcinogenesis Data of A Predominantly Negative Nature Drug-Induced Hematologic Toxicity Embryological Pathology High Throughput Pathology Historical Data of Experimental Animals Immunohistochemical Analysis Molecular Pathology Nomenclature of Lesions Non-mammal Toxicity Study Result or Lesion Induced by Chemicals of Which Names Hidden on Account of the Authors Technology and Methodology Related to Toxicological Pathology Tumor Pathology; Neoplasia and Hyperplasia Ultrastructural Analysis Use of Animal Models.