合成cry1Ab基因的组成表达赋予了马铃薯块茎蛾(Phthorimaea operculella Zeller)幼虫的抗性

IF 1.5 4区农林科学 Q2 Agricultural and Biological Sciences

Crop Breeding and Applied Biotechnology Pub Date : 2021-05-21 DOI:10.1590/1984-70332021V21N1A9

H. Salehian, H. Rahnama, S. Dezhsetan, S. Babaei

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引用次数: 1

摘要

摘要马铃薯块茎蛾是一种破坏性的马铃薯害虫。苏云金芽孢杆菌cry基因编码对PTM具有毒性特征的晶体蛋白。本研究的目的是利用合成的Bt cry1Ab基因培育抗PTM的马铃薯品种Agria。将cry1Ab基因插入pBI121二元表达载体中构建转化载体。将携带pBI35SCry1Ab构建体的根癌农杆菌菌株AGL1用于马铃薯转化。结果表明，在97个假定的转化系中，有30个系为PCR阳性。Southern印迹分析显示，将一到三个拷贝的转基因插入转基因系中。免疫测定显示Cry蛋白的表达。此外，生物测定结果证实了新生幼虫的高死亡率。本研究结果表明，转基因株系在害虫防治方面具有显著的潜力。

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Constitutive expression of a synthetic cry1Ab gene confers resistance to potato tuber moth (Phthorimaea operculella Zeller) larva

Abstract The potato tuber moth (Phthorimaea operculella Zeller) (PTM) is a destructive potato pest. Bacillus thuringiensis cry genes encode crystal proteins that have toxic characteristics against PTM. The aim of the present study was to develop a PTM resistant potato cv. Agria using the synthetic Bt cry1Ab gene. The transformation vector was constructed by inserting the cry1Ab gene into the pBI121 binary expression vector. The Agrobacterium tumefaciens strain AGL1 harboring the pBI35SCry1Ab construct was used for potato transformation. The results indicated that out of 97 putative transformed lines, 30 lines were PCR positive. Southern blot analysis revealed that one to three copies of transgenes were inserted into transgenic lines. The immunoassay test revealed the expression of the Cry protein. Furthermore, the results of bioassays confirmed the high mortality level of neonate larvae. The results of this study suggested that the transgenic lines have significant potential in pest control.

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来源期刊

Crop Breeding and Applied Biotechnology 农林科学-农艺学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.