edulis异门（A.St.-Hil.等）的多酚含量和抗氧化潜力。前Niederl。和金龟子。（智齿目）

IF 0.6 4区农林科学 Q4 FORESTRY

Revista Arvore Pub Date : 2021-07-02 DOI:10.1590/1806-908820210000007

A. Sobottka, Elisandra Tessaro, S. M. Silva, Marina Pedron, Lara Tortini Seffrin

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引用次数: 0

摘要

摘要：紫外异门（Allophylus edulis）和紫柏（Cupania vernalis）是巴西本土的树木，被用作治疗呼吸、消化、循环和皮肤疾病的药用植物。这些物种普遍分布在巴西境内，适合用于旨在恢复退化生态系统的混合种植园。在本研究中，评估了从A.edulis和C.vernalis叶中提取的提取物和组分的总酚含量（TPC）和总黄酮含量（TFC）以及抗氧化活性。用分光光度法测定TPC和TFC。通过2，2-二苯基-1-苦基肼（DPPH）的自由基清除活性来评价其抗氧化活性。通过两种方法获得提取物：浸渍法（方法1）和索氏法（方法2）。使用极性增加的溶剂（己烷、二氯甲烷、乙酸乙酯和正丁醇）来获得级分。结果表明，通过浸渍法获得的A.edulis的乙酸乙酯组分具有最高的TPC（442.0±18.2 mg GAE g-1）和TFC（58.1±0.4 mg RUE g-1），以及抗氧化活性（EC50=43.6±2.6µg mL-1）。通过C.vernalis，正丁醇部分获得了优异的结果（TPC=126.1±5.8 mg GAE g-1，TFC=3.77±0.6 mg RUE g-1）。在粗水醇提取物（EC50=816.1±50.9µg mL-1）和丁醇部分（1156.4±3.8µg mL-2）中发现了最高的抗氧化潜力。本研究的结果表明，用极性更强的溶剂（乙酸乙酯和正丁醇）浸渍和液-液分配得到的馏分中TPC和TFC含量最丰富，并表现出更高的抗氧化活性。比较这两种植物，A.edulis表现出最好的效果，具有高含量的TPC、TFC和抗氧化潜力，因此可以用于治疗与氧化应激相关的疾病。

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POLYPHENOL CONTENT AND ANTIOXIDANT POTENTIAL OF Allophylus edulis (A. St.-Hil. et al.) Hieron. ex Niederl. AND Cupania vernalis Cambess. (SAPINDACEAE)

ABSTRACT Allophylus edulis and Cupania vernalis (Sapindaceae) are Brazilian native trees used as medicinal plants for the treatment of respiratory, digestive, circulatory, and skin diseases. Ubiquitously distributed in the Brazilian territory, these species are indicated for mixed plantations aimed at the recovery of degraded ecosystems. In this study, the total phenolic content (TPC) and total flavonoid content (TFC), and the antioxidant activity of extracts and fractions obtained from A. edulis and C. vernalis leaves were assessed. The TPC and TFC was determined spectrophotometrically. Antioxidant activity was evaluated through radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts were obtained by two methods: maceration (method 1) and Soxhlet (method 2). Solvents of increasing polarity (hexane, dichloromethane, ethyl acetate, and n-butanol) were used to obtained the fractions. The results showed that the ethyl acetate fraction from A. edulis, obtained from the maceration method, had the highest TPC (442.0 ± 18.2 mg GAE g-1) and TFC (58.1 ± 0.4 mg RUE g-1), and antioxidant activity (EC50 = 43.6 ± 2.6 µg mL-1). By C. vernalis, superior results were obtained with the n-butanol fraction (TPC = 126.1 ± 5.8 mg GAE g-1, TFC = 37.7 ± 0.6 mg RUE g-1). The highest antioxidant potential was found in the crude hydroalcoholic extract (EC50 = 816.1 ± 50.9 µg mL-1) and butanol fraction (1,156.4 ± 3.8 µg mL-1). The results of this study show that the fractions obtained by maceration and liquid-liquid partition with more polar solvents (ethyl acetate and n-butanol) are the richest in TPC and TFC, and presented the greater antioxidant activity. Comparing the two plants, A. edulis showed the best results, with a high content of TPC, TFC, and antioxidant potential, and therefore may be used to treat diseases related to oxidative stress.

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Revista Arvore FORESTRY-

CiteScore

1.00

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0.00%

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审稿时长

4-8 weeks

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