诊断新冠肺炎：检测SARS-CoV-2基因组E基因的诊断重要性

IF 2.1 4区医学 Q3 VIROLOGY

Future Virology Pub Date : 2023-01-10 DOI:10.2217/fvl-2021-0330

I. Barjaktarović, J. Maletić, N. Vučinić, A. Milutinović, M. Grujicic, V. Čabarkapa

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引用次数: 1

摘要

目的：评价除N和RdRp基因外的E基因分析对严重急性呼吸系统综合征冠状病毒2型的意义，并比较靶点的特异性和敏感性。材料与方法：我们使用了两种逆转录PCR检测：一种针对N、E和RdRp基因，另一种则针对N和RdRp基因，并分析了阈值周期（Ct）值的变化。结果：在155份样本中，70.32%的样本检测呈阳性：三种基因均检测出45.87%，N和RdRp检测出19.27%，仅N检测出34.86%。结论：E基因阴性的样本可能是假阳性，在解释结果时应评估临床情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Diagnosing COVID-19: diagnostic importance of detecting E gene of the SARS-CoV-2 genome

Aim: To evaluate the significance of E gene analysis in addition to N and RdRp genes of SARS-CoV-2, and to compare the specificity and sensitivity of targets. Materials & methods: We used two reverse transcription-PCR assays: one targeting N, E and RdRp and the other targeting N and RdRp genes and analyzed variation in threshold cycle (Ct) values. Results: Of the 155 samples, 70.32% tested positive: all three genes were detected in 45.87%, N and RdRp in 19.27% and only N in 34.86%. Patients negative for the E gene were tested after symptoms disappeared and Ct values were significantly higher. Conclusion: Samples negative for the E gene were potentially false positive and clinical conditions should be assessed while interpreting results.

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来源期刊

Future Virology 医学-病毒学

CiteScore

4.00

自引率

3.20%

发文量

审稿时长

6-12 weeks

期刊介绍： Future Virology is a peer-reviewed journal that delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this ever-expanding area of research. It is an interdisciplinary forum for all scientists working in the field today.